QUALITY OF LANDRACE PIG LIQUID SEMEN DURING THE COLD SAVE PROCESS USING BTS AND TRIS AMINOMETHANE / DILUENTS EGG YOLK 20% ON 2-5C

Abstract

Landrace pigs are often found in East Nusa Tenggara, which can fulfill the community's protein needs and Artificial Insemination can be used to improve the genetic quality of pigs. The aim of this study was to determine the shelf life of the quality of the spermatozoa of Landrace pigs using BTS thinners and tris aminomethane + egg yolk 20%. This research was conducted at the University of Timor Agriculture Faculty Laboratory using Landrace pig semen. The research method was laboratory experiments using 2 treatments with 10 replications, namely T1 (BTS) and T2 (Tris Aminomethan + egg yolk 20%) which were stored at refrigerator temperature 2-5oC. The variables observed were individual motility and total motile spermatozoa. Data were analyzed using Randomized Block Design (RBD). The percentage of motility and total motile sperm were tested using Pearson's Chi Square with an expected value of 40 million motile sperm / ml. The results showed that the length of storage for the 3 hours of thinners did not have a significant effect (P> 0.05) while the storage time for the 6th hour, 9th hour, 24.48 and 72 percent motility of T1 individuals with 66.5%, 61.5%, 52.5 %, 42.5%, 32% were significantly different (P <0.05) towards and T2 was 63%, 57% 47%, 37%, 22.5%. The conclusion of this study is that BTS thinners have the ability to maintain the percentage of individual motility longer than Tris Aminomethane + egg yolk 20%.

Keywords

• Liquid semen
• landrace pig
• individual motility
• total motile spermatozoa
• beltsville thawing solution