An Integrated Theranostic Nanomaterial for Targeted Photodynamic Therapy of Infectious Endophthalmitis

Yingying Jin; Yuqin Wang; Jie Yang; Hengrui Zhang; Ying-Wei Yang; Wei Chen; Wenya Jiang; Jia Qu; Yishun Guo; Bailiang Wang

Cell Reports Physical Science (Aug 2020)

An Integrated Theranostic Nanomaterial for Targeted Photodynamic Therapy of Infectious Endophthalmitis

Yingying Jin,
Yuqin Wang,
Jie Yang,
Hengrui Zhang,
Ying-Wei Yang,
Wei Chen,
Wenya Jiang,
Jia Qu,
Yishun Guo,
Bailiang Wang

Affiliations

Yingying Jin: Research Laboratory of Advanced Ophthalmic Biomaterials and Drug Delivery, School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou 325027, P.R. China
Yuqin Wang: Research Laboratory of Advanced Ophthalmic Biomaterials and Drug Delivery, School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou 325027, P.R. China
Jie Yang: State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, International Joint Research Laboratory of Nano-Micro Architecture Chemistry (NMAC), College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130012, P.R. China
Hengrui Zhang: Research Laboratory of Advanced Ophthalmic Biomaterials and Drug Delivery, School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou 325027, P.R. China
Ying-Wei Yang: State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, International Joint Research Laboratory of Nano-Micro Architecture Chemistry (NMAC), College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130012, P.R. China; Corresponding author
Wei Chen: Research Laboratory of Advanced Ophthalmic Biomaterials and Drug Delivery, School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou 325027, P.R. China
Wenya Jiang: Research Laboratory of Advanced Ophthalmic Biomaterials and Drug Delivery, School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou 325027, P.R. China
Jia Qu: Research Laboratory of Advanced Ophthalmic Biomaterials and Drug Delivery, School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou 325027, P.R. China; Corresponding author
Yishun Guo: Research Laboratory of Advanced Ophthalmic Biomaterials and Drug Delivery, School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou 325027, P.R. China
Bailiang Wang: Research Laboratory of Advanced Ophthalmic Biomaterials and Drug Delivery, School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou 325027, P.R. China; Corresponding author

Journal volume & issue: Vol. 1, no. 8
p. 100173

Abstract

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Summary: Infectious endophthalmitis is a growing concern that causes irreversible intraocular tissue and optic nerve damage. Here, we report a theranostic nanoplatform based on UiO-66-NH2 to combine photodynamic therapy (PDT) and lipopolysaccharide (LPS) targeting through polypeptide (YVLWKRKRKFCFI-NH2) modification. Toluidine blue (TB), serving as a photosensitizer (PS), is loaded into UiO-66-NH2 nanoparticles (NPs) for both self-imaging and PDT functions. The accelerated TB release at pH 5.5 compared to pH 7.4 demonstrates the responsive drug release feature. In vitro antibacterial tests, including contact incubation-agar plate counting, bacterial live/dead staining, and scanning electron microscopy observations, confirm the utility of the TB@UiO-66-NH2@PEP&PEG NPs (UTPP NPs) in biofilms removal and sterilization of Pseudomonas aeruginosa and Staphylococcus epidermidis. Furthermore, the UTPP NPs also show good biocompatibility toward human retinal pigment epithelial cells, as well as in situ self-imaging property. Finally, an in vivo infectious endophthalmitis model supports the application of the UTPP NPs in infectious endophthalmitis treatment.

Published in Cell Reports Physical Science

ISSN: 2666-3864 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Physics
Website: https://www.cell.com/cell-reports-physical-science

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