O4 Effect of INGAP-PP on the expression of genes involved in extracellular matrix remodeling and angiogenesis determined by a transcriptomic analysis in rat islets.

Abstract

Introduction: INGAP-PP is a peptide derived from INGAP that, in normal and diabetic animals, increases β cell mass, insular angiogenic capacity and enhances insulin secretion stimulated by glucose and other secretagogues. This makes it a therapeutic tool that would act specifically in the cellular pathogenesis of diabetes. Knowing its possible effect on global changes in insular gene expression profiles would facilitate the understanding of its mechanism of action. Aim: to know, through a transcriptomic study, the effect of INGAP-PP on islet gene expression and the possible regulation of different signaling pathways involved. Materials and Methods: Isolated islets (collagenase) of normal Wistar rats were cultured for 4 days in RPMI medium in the absence (C) or presence of INGAP-PP 50 µg / ml (I) and glucose 11 mM. At the end, 100 islets from each group were used to study insulin secretion at 3.3 mM or 16.6 mM glucose (RIA). Total RNA was extracted from the rest of the islets, one aliquot was subjected to massive RNA sequencing (RNA-seq) and the other was used to measure gene expression level by RT-qPCR of key genes modulated by INGAP-PP involved in development, regeneration, and angiogenesis pathways.

Keywords

• ingap-pp
• remodelación de la matriz extracelular
• angiogénesis