Arboviruses in Free-Ranging Birds and Hematophagous Arthropods (Diptera, Nematocera) from Forest Remnants and Urbanized Areas of an Environmental Protection Area in the Amazon Biome

Bruna Alves Ramos; Liliane Leal Das Chagas; Franko de Arruda e Silva; Eder Barros dos Santos; Jannifer Oliveira Chiang; Joaquim Pinto Nunes Neto; Durval Bertram Rodrigues Vieira; José Wilson Rosa Junior; Eliana Vieira Pinto da Silva; Maria Nazaré Oliveira Freitas; Maissa Maia Santos; Jamilla Augusta de Sousa Pantoja; Ercília de Jesus Gonçalves; Landeson Junior Leopoldino Barros; Sandro Patroca Silva; Carine Fortes Aragão; Ana Cecilia Ribeiro Cruz; Lívia Medeiros Neves Casseb; Lizandra Caroline dos Santos Souto; Joana D’Arc Pereira Mascarenhas; Erilene Cristina Da Silva Furtado; Raimundo Nelson Souza Da Silva; Alexandre do Rosário Casseb; Lívia Carício Martins

doi:10.3390/v14102101

Viruses (Sep 2022)

Arboviruses in Free-Ranging Birds and Hematophagous Arthropods (Diptera, Nematocera) from Forest Remnants and Urbanized Areas of an Environmental Protection Area in the Amazon Biome

Bruna Alves Ramos,
Liliane Leal Das Chagas,
Franko de Arruda e Silva,
Eder Barros dos Santos,
Jannifer Oliveira Chiang,
Joaquim Pinto Nunes Neto,
Durval Bertram Rodrigues Vieira,
José Wilson Rosa Junior,
Eliana Vieira Pinto da Silva,
Maria Nazaré Oliveira Freitas,
Maissa Maia Santos,
Jamilla Augusta de Sousa Pantoja,
Ercília de Jesus Gonçalves,
Landeson Junior Leopoldino Barros,
Sandro Patroca Silva,
Carine Fortes Aragão,
Ana Cecilia Ribeiro Cruz,
Lívia Medeiros Neves Casseb,
Lizandra Caroline dos Santos Souto,
Joana D’Arc Pereira Mascarenhas,
Erilene Cristina Da Silva Furtado,
Raimundo Nelson Souza Da Silva,
Alexandre do Rosário Casseb,
Lívia Carício Martins

Affiliations

Bruna Alves Ramos: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, km 07, s/n, Levilândia, Ananindeua 67030-000, PA, Brazil
Liliane Leal Das Chagas: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, km 07, s/n, Levilândia, Ananindeua 67030-000, PA, Brazil
Franko de Arruda e Silva: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, km 07, s/n, Levilândia, Ananindeua 67030-000, PA, Brazil
Eder Barros dos Santos: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, km 07, s/n, Levilândia, Ananindeua 67030-000, PA, Brazil
Jannifer Oliveira Chiang: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, km 07, s/n, Levilândia, Ananindeua 67030-000, PA, Brazil
Joaquim Pinto Nunes Neto: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, km 07, s/n, Levilândia, Ananindeua 67030-000, PA, Brazil
Durval Bertram Rodrigues Vieira: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, km 07, s/n, Levilândia, Ananindeua 67030-000, PA, Brazil
José Wilson Rosa Junior: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, km 07, s/n, Levilândia, Ananindeua 67030-000, PA, Brazil
Eliana Vieira Pinto da Silva: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, km 07, s/n, Levilândia, Ananindeua 67030-000, PA, Brazil
Maria Nazaré Oliveira Freitas: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, km 07, s/n, Levilândia, Ananindeua 67030-000, PA, Brazil
Maissa Maia Santos: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, km 07, s/n, Levilândia, Ananindeua 67030-000, PA, Brazil
Jamilla Augusta de Sousa Pantoja: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, km 07, s/n, Levilândia, Ananindeua 67030-000, PA, Brazil
Ercília de Jesus Gonçalves: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, km 07, s/n, Levilândia, Ananindeua 67030-000, PA, Brazil
Landeson Junior Leopoldino Barros: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, km 07, s/n, Levilândia, Ananindeua 67030-000, PA, Brazil
Sandro Patroca Silva: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, km 07, s/n, Levilândia, Ananindeua 67030-000, PA, Brazil
Carine Fortes Aragão: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, km 07, s/n, Levilândia, Ananindeua 67030-000, PA, Brazil
Ana Cecilia Ribeiro Cruz: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, km 07, s/n, Levilândia, Ananindeua 67030-000, PA, Brazil
Lívia Medeiros Neves Casseb: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, km 07, s/n, Levilândia, Ananindeua 67030-000, PA, Brazil
Lizandra Caroline dos Santos Souto: Department of Virology, Evandro Chagas Institute, BR 316, km 07, s/n, Levilândia, Ananindeua 67030-000, PA, Brazil
Joana D’Arc Pereira Mascarenhas: Department of Virology, Evandro Chagas Institute, BR 316, km 07, s/n, Levilândia, Ananindeua 67030-000, PA, Brazil
Erilene Cristina Da Silva Furtado: Central Laboratory of Pará State (LACEN-PA), Boulevard Augusto Montenegro, 524, Guajará Park, Belem 66823-010, PA, Brazil
Raimundo Nelson Souza Da Silva: Health and Animal Production Institute, Federal Rural University of Amazonia, Boulevard Pres. Tancredo Neves, 2501, Terra Firme, Belem 66077-830, PA, Brazil
Alexandre do Rosário Casseb: Health and Animal Production Institute, Federal Rural University of Amazonia, Boulevard Pres. Tancredo Neves, 2501, Terra Firme, Belem 66077-830, PA, Brazil
Lívia Carício Martins: Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, BR 316, km 07, s/n, Levilândia, Ananindeua 67030-000, PA, Brazil

DOI: https://doi.org/10.3390/v14102101
Journal volume & issue: Vol. 14, no. 10
p. 2101

Abstract

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The rapid and disorderly urbanization in the Amazon has resulted in the insertion of forest fragments into cities, causing the circulation of arboviruses, which can involve hematophagous arthropods and free-ranging birds in the transmission cycles in urban environments. This study aimed to evaluate the circulation of arboviruses in free-ranging birds and hematophagous arthropods captured in an Environmental Protection Area in the Belem metropolitan area, Brazil. Birds were captured using mist nets, and hematophagous arthropods were collected using a human protected attraction technique and light traps. The birds’ sera were subjected to a hemagglutination inhibition test to detect antibodies against 29 arbovirus antigens. Arthropod macerates were inoculated into C6/36 and VERO cell cultures to attempt viral isolation and were tested using indirect immunofluorescence, subsequent genetic sequencing and submitted for phylogenetic analysis. Four bird sera were positive for arbovirus, and one batch of Psorophora ferox was positive for Flavivirus on viral isolation and indirect immunofluorescence. In addition, the Ilheus virus was detected in the sequencing and phylogenetic analysis. The presence of antibodies in sera from free-ranging birds and the isolation of Ilheus virus in Psorophora ferox indicate the circulation of arboviruses in forest remnants in the urban center of Belem.

Published in Viruses

ISSN: 1999-4915 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.mdpi.com/journal/viruses

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