OPTIMIZATION OF SURGICAL APPROACH OF TREATMENT IN PATIENTS WITH FULL-THICKNESS THERMAL BURNS

Abstract

OBJECTIVE. The study investigated methods of improving the results of active surgical treatment of patients with severe burns and at the same time the possibility of determination of optimal terms of autografting and shortening the terms of hospital stay. MATERIALS AND METHODS. Researches were carried out in patients aged from 18 to 60 at the period from 2007 to 2015. Immunohistochemical techniques were used to detect an expression of Ki-67 gene and localization of immune cells in dynamics. RESULTS. The authors have obtained the morphological criteria of readiness of burned wound to autografting with following prediction of results. The graft healing processes depended on angiogenesis activity and quantity of functioning blood vessels in the burn wound and at its border of intact skin. The regenerative potential of tissue structures in the burn area and at its border was determined by the expression of Ki67gene. These data allowed the authors to forecast the outcome of skin grafting. CONCLUSIONS. The optimal ratio of immune cells in burn wound at its border with intact skin is a criterion of readiness for autodermoplasty. Autodermoplasty should have been performed in terms of 9 days since the moment of burn injury in order to have a significant advantage compared with subsequent terms of grafting.

Keywords

• burns
• skin
• keratinocytes
• effector immunocytes
• immune homeostasis
• autodermoplasty
• reparative