Extraction and Purification of Exopolysaccharides from Exhausted Arthrospira platensis (Spirulina) Culture Systems

Abstract

Microalgal endo and exopolysaccharides (EPS) are attracting increasing interest for their potential applications in the food, cosmetic and pharmaceutical industries. The standard applications of microbial EPS are as food coatings, emulsifying and gelling agents, flocculants, hydrating agents etc. They present unique biochemical properties that make them interesting from the biotechnological point of view. Their physical-chemical properties are interesting for biomedical applications, since polysaccharides have been demonstrated to possess inhibitory properties against various types of viruses, bacteria and tumors. The purpose of this work is to upgrade the exhausted culture media resulting from the cultivation of the cyanobacterium Arthrospira platensis (Spirulina), in order to extract the exopolysaccharides excreted by the cyanobacterium and test their exploitation potential in a cosmetic context (a body cream). The study results include: defining the composition and the productivity of EPS by the Spirulina culture, developing a suitable application method for the DPPH assay in lipophilic matrices, and evaluation of the antioxidant action of these polymers in the cosmetic field.