Zhongguo youzhi (Aug 2023)
栾树籽油对非酒精性脂肪肝的保护作用Protection effect of Koelreuteria paniculata seed oil on non- alcoholic fatty liver disease
Abstract
为了促进栾树资源的开发和利用，通过体外细胞实验和体内动物实验研究了栾树籽油对非酒精性脂肪肝（NAFLD）的保护作用。体外实验采用游离脂肪酸（FFA）诱导人正常肝细胞系Lo-2，建立NAFLD细胞模型，观察栾树籽油的干预对Lo-2细胞脂肪变性以及甘油三酯（TG）含量的影响，观察栾树籽油对NAFLD细胞脂质堆积的影响。体内实验采用高脂饲料饲养诱导建立NAFLD小鼠模型，造模成功后连续灌胃给药7 d，取小鼠血清、肝脏组织，观察肝脏组织病理学，检测血清及肝脏组织总胆固醇（TC）、TG、谷草转氨酶（AST）和谷丙转氨酶（ALT）水平，探讨栾树籽油对NAFLD小鼠肝脏的保护作用。结果显示：通过FFA诱导Lo-2建立NAFLD细胞模型，栾树籽油干预NAFLD细胞24 h后，Lo-2细胞增殖能力增强，显示出一定的细胞保护能力，油红O染色结果表明栾树籽油能显著减少FFA诱导的Lo-2细胞内脂质堆积，降低TG的含量，且呈剂量依赖性；NAFLD小鼠实验表明栾树籽油具有降低血脂、改善脂质沉积和保护肝脏的作用。综上，体内外实验均证明栾树籽油可减少细胞内脂质的积累，从而达到降脂效果。In order to promote the development and utilization of Koelreuteria paniculata plant resources, the protection effect of Koelreuteria paniculata seed oil against non-alcoholic fatty liver disease（NAFLD） was investigated through in vitro cellular experiments and in vivo animal experiments. In vitro experiments were conducted using free fatty acid (FFA)-induced human hepatocytes (Lo-2) to establish a NAFLD cell model to observe the effects of Koelreuteria paniculata seed oil intervention on steatosis and triglyceride (TG) content in Lo-2 cells, and to observe the effect of Koelreuteria paniculata seed oil on lipid accumulation in the NAFLD cell model. Through in vivo experiments, a mice model of NAFLD was established using high-fat diet feeding. After successful modelling, the mice were administered continuously with Koelreuteria paniculata seed oil by gavage for 7 d. Serum and liver tissues of the mice were collected to observe liver histopathology, and the levels of total cholesterol (TC), TG, glutamic transaminase (AST) and glutamic aminotransferase (ALT) were measured to investigate the hepatoprotective effects of Koelreuteria paniculata seed oil on mice with NAFLD. The results showed that NAFLD cell models were established by FFA induction of Lo-2, and after Koelreuteria paniculata seed oil intervened in the model cells for 24 h, the proliferation ability of Lo-2 cells was enhanced and they showed certain cytoprotective ability. The results of oil red O staining showed that Koelreuteria paniculata seed oil could significantly reduce the accumulation of lipids in FFA-induced Lo-2 cells and lower the content of TG, and the effect was concentration-dependent. Experiments on NAFLD mice showed that Koelreuteria paniculata seed oil could lower blood lipids, improve lipid accumulation, and protect the liver. In conclusion, from in vivo and in vitro experiments, Koelreuteria paniculata seed oil can reduce the accumulation of intracellular lipids, thus achieving a lipid-lowering effect.
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