<b>Utilization of low-cost substrates for the production of nystose by <i>Bacillus subtilis</i> natto cct 7712

Abstract

Current analysis describes the capacity of Bacillus subtilis natto CCT 7712 to produce high amounts of nystose by low-cost substrates available in Brazil, such as commercial sucrose, sugarcane molasses and sugarcane juice. Optimization resulted in a maximum production of 179.77 g L-1 of nystose, averaging 7.49 g L-1 hour-1 of productivity and a 71.73% yield in a medium with 400 g L-1of commercial sucrose and 0.8 g L-1 of MnSO4. Fermentations with sugarcane molasses and sugarcane juice also resulted in a satisfactory production reaching 97.93 and 42.58 g L-1 nystose, respectively. High nystose production in a medium with sugarcane derivatives suggests submerged fermentation with Bacillus subtillis natto CT 7712 as a promising strategy to produce nystose at industrial level.

Keywords

• Bacillus subtilis natto CT 7712
• commercial sucrose
• nystose
• sugarcane juice
• sugarcane molasses