Investigation methods of adhesion of microorganisms.

Abstract

Background. Adhesion is the main property of microorganisms that determines its pathogenicity. Laboratory examination of adhesion is important to determine the virulence of the bacteria and its effect on the development of pathology. Objective. Compilation of the main research methods of microbal adhesion and their possible use in common laboratory diagnostics. Results. Microbal adhesion is explored in vivo and in vitro. Research in vivo requires the native biological models. For example, studies of adhesion of S. aureus relatively a rats’ blood vessels when fluorochrome labeled bacteria enter the body of biological models and watch the number of microorganisms adherent to the arterial walls of the peritoneum, previously performed surgical access. The scientists used fluorescent microscopy for this. Research methods of microbal adhesion in vitro include the study of the adhesive properties of red blood cells using Group 1 (O) Rh +; research using adhesive properties of mammals’ red blood cells; study the microbal adhesion to hemoprotein, fibronectin and fibrinogen; evaluation of adhesive properties by Photocolorimetry; measuring forces acting between S. aureus and human skin. Conclusion. The most accurate and easy-to-carrying is a research method of adhesion as Photocolorimetry. It’s very important to use the definition of microorganism adhesive properties not only for scientific purpose, but also in usual diagnostics of infectious diseases.

Keywords

• адгезія
• вірулентність
• патогенність
• фотоколориметрія
• діагностика інфекційних захворювань