A SALTING-OUT ASSISTED LIQUID-LIQUID EXTRACTION FOR THE DETERMINATION OF TEBUCONAZOLE, CARBOFURAN AND IMIDACLOPRID IN WHITE AND ROSÉ WINES

Abstract

Food safety is a constant and necessary concern due to the worldwide increase of the food demand. The monitoring of quality parameters demands the development of accurate, easy and fast procedures. The aim of this work was to develop a cheap, miniaturized and trustworthy salting-out assisted liquid-liquid extraction (SALLE) procedure for the determination of pesticides in wines. Sample and acetonitrile were mixed and the addition of NaCl and CH3COONa were used for separation of aqueous and organic phases. After centrifugation, the organic phase was directly injected into a liquid chromatograph. The developed SALLE procedure consumed at least 25-fold less reagent compared to conventional QuEChERS procedure. Additionally, the d-SPE step of the QuEChERS method was suppressed, without efficiency losses. The developed procedure was compared to the conventional methodology and no significant difference was observed. It was successfully applied to the determination of three pesticides in wines by liquid chromatography. Low consumption of sample and solvent, low residues generation, simple instrumentation are notable advantages. The suppression of the d-SPE step simplified the process and the risk of analyte losses.

Keywords

• pesticides
• liquid chromatography
• sample preparation