Decorin Promotes Osteoblastic Differentiation of Human Periodontal Ligament Stem Cells

Orie Adachi; Hideki Sugii; Tomohiro Itoyama; Shoko Fujino; Hiroshi Kaneko; Atsushi Tomokiyo; Sayuri Hamano; Daigaku Hasegawa; Junko Obata; Shinichiro Yoshida; Masataka Kadowaki; Risa Sugiura; Mhd Safwan Albougha; Hidefumi Maeda

doi:10.3390/molecules27238224

Molecules (Nov 2022)

Decorin Promotes Osteoblastic Differentiation of Human Periodontal Ligament Stem Cells

Orie Adachi,
Hideki Sugii,
Tomohiro Itoyama,
Shoko Fujino,
Hiroshi Kaneko,
Atsushi Tomokiyo,
Sayuri Hamano,
Daigaku Hasegawa,
Junko Obata,
Shinichiro Yoshida,
Masataka Kadowaki,
Risa Sugiura,
Mhd Safwan Albougha,
Hidefumi Maeda

Affiliations

Orie Adachi: Department of Endodontology and Operative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan
Hideki Sugii: Department of Endodontology and Operative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan
Tomohiro Itoyama: Department of Endodontology, Kyushu University Hospital, Fukuoka 812-8582, Japan
Shoko Fujino: Department of Endodontology and Operative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan
Hiroshi Kaneko: Department of Endodontology, Kyushu University Hospital, Fukuoka 812-8582, Japan
Atsushi Tomokiyo: Department of Endodontology, Kyushu University Hospital, Fukuoka 812-8582, Japan
Sayuri Hamano: Department of Endodontology and Operative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan
Daigaku Hasegawa: Department of Endodontology, Kyushu University Hospital, Fukuoka 812-8582, Japan
Junko Obata: Department of Endodontology and Operative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan
Shinichiro Yoshida: Department of Endodontology, Kyushu University Hospital, Fukuoka 812-8582, Japan
Masataka Kadowaki: Department of Endodontology and Operative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan
Risa Sugiura: Department of Endodontology and Operative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan
Mhd Safwan Albougha: Department of Endodontology and Operative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan
Hidefumi Maeda: Department of Endodontology and Operative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan

DOI: https://doi.org/10.3390/molecules27238224
Journal volume & issue: Vol. 27, no. 23
p. 8224

Abstract

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The aim of this study is to clarify the biological functions of decorin (DCN) in the healing and regeneration of wounded periodontal tissue. We investigated the expression pattern of DCN during the healing of wounded periodontal tissue in rats by immunohistochemistry and the effects of DCN on the osteoblastic differentiation of human periodontal ligament (PDL) stem cells (HPDLSCs) and preosteoblasts by Alizarin red S staining, quantitative reverse transcription-polymerase chain reactions, and western blotting. The expression of DCN was increased around the wounded PDL tissue on day 5 after surgery compared with the nonwounded PDL tissue, whereas its expression was not changed in the osteoblastic layer around the wounded alveolar bone. Furthermore, DCN promoted the osteoblastic differentiation of HPDLSCs, but it did not affect the osteoblastic differentiation of preosteoblasts. ERK1/2 phosphorylation was upregulated during the DCN-induced osteoblastic differentiation of HPDLSCs. DCN did not affect proliferation, migration, or the PDL-related gene expression of HPDLSCs. In conclusion, this study demonstrates that DCN has a role in the healing of wounded periodontal tissue. Furthermore, DCN secreted from PDL cells may contribute to bone healing by upregulating osteoblastic differentiation through ERK1/2 signaling in HPDLSCs, indicating a therapeutic effect of DCN in periodontal tissue regeneration.

Published in Molecules

ISSN: 1420-3049 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Organic chemistry
Website: http://www.mdpi.com/journal/molecules

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