Evaluation of (cytotoxicity and angiogenesis) of Zinc oxide nanoparticles Synthesized by aqueous extract of Origanum majorana

Abstract

Background and Aim: Zinc oxide nanoparticle (ZnO NPs), Due to its unique properties, such as its anti-cancer and antioxidant properties, it has attracted researchers attention. In the present study, the cytotoxic and anti-angiogenic properties of zinc oxide nanoparticles prepared with the green method from extract Origanum Majorana were investigated. Materials and Methods: Cytotoxic effects of zinc oxide nanoparticles, By MTT assay on cells of HUVEC، MDA-MB-231 and Hep-G2 was examined. In order to evaluate the anti-angiogenic effects in an in vivo model, 48 fertile eggs with Ross breed randomly divided into 6 groups Consist of: 4 treatment groups with different concentrations of (125, 250, 500 and 1000 µg/ml), control and sham groups. After 12 days of incubation, the number and the length of the vessels of the chorioallantoic membrane of chick embryos were assessed. Eventually, data were analyzed using Image J and SPSS software (version 16) and one-way ANOVA and LSD statistical tests at the significant level (P<0.05). Results: The results showed that ZnO nanoparticles made the concentration-dependent cytotoxic effects with the amount IC50 values ​​of 60, 15 and 80 μg/ml. The number and length of chorioallantoic membrane vessels and by chick embryos weight and height in treated groups with different concentrations of nanoparticles significantly decreased compared to the control group (p<0.05). Conclusion: The Zinc oxide nanoparticles produced by the green method from the extract of Marjoram probably with more severe cytotoxic properties for cancerous cells compared to healthy cells and it can also have anti-angiogenic potential.

Keywords

• angiogenesis
• chorioallantoic membrane
• cytotoxicity
• origanum majorana
• zno-nps