STAR Protocols (Mar 2023)

Protocol for aerosolization challenge of mice with Bordetella pertussis

  • Graham Bitzer,
  • Kelly Weaver,
  • Mariette Barbier,
  • F. Heath Damron

Journal volume & issue
Vol. 4, no. 1
p. 101979

Abstract

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Summary: Bordetella pertussis causes whooping cough and is transmitted via respiratory droplets. Here, we present a protocol to challenge mice with Bordetella pertussis. We describe bacteria preparation and long-term storage, followed by manufacturing a challenge dose for use in a commercial exposure chamber with controlled nebulization of B. pertussis into aerosols. We then detail the aerosol challenge of mice through a more natural administration than intranasal instillation and post-challenge data collection. This protocol allows for better comparisons between preclinical pertussis studies. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

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