MethodsX (Jun 2024)

Step-by-step approach: Stereotaxic surgery for in vivo extracellular field potential recording at the rat Schaffer collateral-CA1 synapse using the eLab system

  • Mansour Azimzadeh,
  • Mohd Amirul Najwa Mohd Azmi,
  • Parham Reisi,
  • Pike-See Cheah,
  • King-Hwa Ling

Journal volume & issue
Vol. 12
p. 102544

Abstract

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In vivo extracellular field potential recording is a commonly used technique in modern neuroscience research. The success of long-term electrophysiological recordings often depends on the quality of the implantation surgery. However, there is limited use of visually guided stereotaxic neurosurgery and the application of the eLab/ePulse electrophysiology system in rodent models. This study presents a practical and functional manual guide for surgical electrode implantation in rodent models using the eLab/ePulse electrophysiology system for recording and stimulation purposes to assess neuronal functionality and synaptic plasticity. The evaluation parameters included the input/output function (IO), paired-pulse facilitation or depression (PPF/PPD), long-term potentiation (LTP), and long-term depression (LTD). • Provides a detailed picture-guided procedure for conducting in vivo stereotaxic neurosurgery. • Specifically covers the insertion of hippocampal electrodes and the recording of evoked extracellular field potentials.

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