Probable Role of Bilophila wadsworthia in Appendicle Infection

Abstract

ABSTRACT Background and Objective: Bilophlia spp. are gram-negative, pleomorphic rod, obligate anaerobe, oxidase-negative, catalase-positive and non-motile bacteria. B. wadsworthia is type species of genus Bilophila with the additional characteristic of urea hydrolysis. B. wadsworthia can be found in a variety of anaerobe infections, particularly appendicitis and intra-abdominal infection that are considered as important opportunistic pathogens. Methods: This study was designed to identify Bilophila spp. in clinical specimens by culture and PCR. We examined 91 DNA samples extracted from infected appendix tissues with specific primers. Results: Data showed that Bilophila spp. DNA existence in 53.85% (n=49) provided appendiceal tissue. Conclusion: The pathological and molecular examination of infected appendiceal tissues revealed that B. wadsworthia is able to act as the primary cause of significant lesions in the appendicle tissues. Key words: Bilophila spp., Appendectomy, Appendicle specimens, PCR, Nucleotide sequencing

Keywords

• bilophila spp.
• appendectomy
• appendicle specimens
• pcr
• nucleotide sequencing