SERS-Based Flavonoid Detection Using Ethylenediamine-β-Cyclodextrin as a Capturing Ligand

Jae Min Choi; Eunil Hahm; Kyeonghui Park; Daham Jeong; Won-Yeop Rho; Jaehi Kim; Dae Hong Jeong; Yoon-Sik Lee; Sung Ho Jhang; Hyun Jong Chung; Eunae Cho; Jae-Hyuk Yu; Bong-Hyun Jun; Seunho Jung

doi:10.3390/nano7010008

Nanomaterials (Jan 2017)

SERS-Based Flavonoid Detection Using Ethylenediamine-β-Cyclodextrin as a Capturing Ligand

Jae Min Choi,
Eunil Hahm,
Kyeonghui Park,
Daham Jeong,
Won-Yeop Rho,
Jaehi Kim,
Dae Hong Jeong,
Yoon-Sik Lee,
Sung Ho Jhang,
Hyun Jong Chung,
Eunae Cho,
Jae-Hyuk Yu,
Bong-Hyun Jun,
Seunho Jung

Affiliations

Jae Min Choi: Center for Biotechnology Research in UBITA (CBRU), Institute for Ubiquitous Information Technology and Application (UBITA), Konkuk University, Seoul 05029, Korea
Eunil Hahm: Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Korea
Kyeonghui Park: Department of Bioscience and Biotechnology, Microbial Carbohydrate Resource Bank (MCRB) & Center for Biotechnology Research in UBITA (CBRU), Konkuk University, Seoul 05029, Korea
Daham Jeong: Department of Bioscience and Biotechnology, Microbial Carbohydrate Resource Bank (MCRB) & Center for Biotechnology Research in UBITA (CBRU), Konkuk University, Seoul 05029, Korea
Won-Yeop Rho: Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Korea
Jaehi Kim: Department of Chemistry Education, Seoul National University, Seoul 08826, Korea
Dae Hong Jeong: Department of Chemistry Education, Seoul National University, Seoul 08826, Korea
Yoon-Sik Lee: School of Chemical and Biological Engineering, Seoul National University, Seoul 08826, Korea
Sung Ho Jhang: Department of Physics, Konkuk University, Seoul 05029, Korea
Hyun Jong Chung: Department of Physics, Konkuk University, Seoul 05029, Korea
Eunae Cho: Center for Biotechnology Research in UBITA (CBRU), Institute for Ubiquitous Information Technology and Application (UBITA), Konkuk University, Seoul 05029, Korea
Jae-Hyuk Yu: Departments of Bacteriology and Genetics, University of Wisconsin-Madison, Madison, WI 53706, USA
Bong-Hyun Jun: Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Korea
Seunho Jung: Center for Biotechnology Research in UBITA (CBRU), Institute for Ubiquitous Information Technology and Application (UBITA), Konkuk University, Seoul 05029, Korea

DOI: https://doi.org/10.3390/nano7010008
Journal volume & issue: Vol. 7, no. 1
p. 8

Abstract

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Ethylenediamine-modified β-cyclodextrin (Et-β-CD) was immobilized on aggregated silver nanoparticle (NP)-embedded silica NPs (SiO2@Ag@Et-β-CD NPs) for the effective detection of flavonoids. Silica NPs were used as the template for embedding silver NPs to create hot spots and enhance surface-enhanced Raman scattering (SERS) signals. Et-β-CD was immobilized on Ag NPs to capture flavonoids via host-guest inclusion complex formation, as indicated by enhanced ultraviolet absorption spectra. The resulting SiO2@Ag@Et-β-CD NPs were used as the SERS substrate for detecting flavonoids, such as hesperetin, naringenin, quercetin, and luteolin. In particular, luteolin was detected more strongly in the linear range 10−7 to 10−3 M than various organic molecules, namely ethylene glycol, β-estradiol, isopropyl alcohol, naphthalene, and toluene. In addition, the SERS signal for luteolin captured by the SiO2@Ag@Et-β-CD NPs remained even after repeated washing. These results indicated that the SiO2@Ag@Et-β-CD NPs can be used as a rapid, sensitive, and selective sensor for flavonoids.

Published in Nanomaterials

ISSN: 2079-4991 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry
Website: https://www.mdpi.com/journal/nanomaterials

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