miR-188 regulates TGF-α to inhibit proliferation and migration of osteosarcoma cells

Abstract

Objective To explore the effects of miR-188 and transforming growth factor α (TGF-α) on the proliferation and migration of osteosarcoma cells. Methods The expressions of miR-188 and TGF-α in human osteosarcoma tissues and adjacent tissues were detected. TGFA was detected as the target gene of miR-188 by TargetScan and dual luciferase reporter gene assay. The migration ability of osteosarcoma cells line MG63 was examined by cell scratch assay. The xenograft model of osteosarcoma was established in nude mice and the effect of miR-188 was observed in a xenograft model of osteosarcoma of nude mice. Results The expression of miR-188 was decreased in osteosarcoma tissues (P＜0.01). miR-188 specifically bound to the 3′-UTR of TGFA and negatively regulated its expression. The transfection of miR-188 mimic could inhibit the migration ability of MG63 cells,while the inhibitor could promoted the migration ability,but the up-regulation of TGF-α and the expression of interfered TGF-α could reduce the inhibition effect. In a nude mouse model of osteosarcoma xenograft,over-expression of miR-188 reduced the inhibitory effect (P＜0.01), while down-regulating the expression of miR-188 expression had the opposite effect. TGF-α expression was significantly increased in osteosarcoma tissues (P＜0.01),while silence protiferation of osteosarcoing was decreased. Conclusions miR-188 may inhibit the proliferation and migration of MG63 cells by targeting the expression of TGF-α.

Keywords

• osteosarcoma|mir-188|tgf-α|metastasis|nude mouse