ANTIOXIDANT ACTIVITY DUAL METHODS OF DIFFERENT POLARITY EXTRACTS OF STEM OF SOLANUM NIGRUM L. GROWING IN ALGERIA

Abstract

The purpose of this study is to evaluate the antioxidants properties and phenolic and flavonoid contents of Solanum nigrum L. growing in El-Oued region (south east of Algeria). The different polarity extracts chloroform (CHCl3), ethyl acetate (AcOEt) and n-butanol (n-BuOH) have been prepared from the aqueous ethanol extract of the stem. The antioxidant activity of extracts was determined by DPPH (2, 2-diphenyl-1-picrylhydrazyl), FRAP (Ferric ion reducing antioxidant power) assays. The results showed that CHCl3 extract has the highest level of polyphenol (66.427 ± 0.001 mg gallic acid / g) while, the AcOEt extract has the highest level of flavonoid content (26.450 ± 0.079 mg rutin equivalents / g). In addition, the highest IC50 of DPPH scavenging activity has exhibited the most potent antioxidant capacity assay of CHCl3 (0.231 mg∙mL-1) compared to the n-BuOH and AcOEt extracts. Furthermore, the highest value of FRAP for AcOEt extract was 458.291 ± 0.005. In this study, it has been noted that the potency of the antioxidant activity of DPPH may be identified through different levels of polyphenol. However, the value of FRAP has a relation to the value of flavonoid.

Keywords

• antioxidants properties
• dpph
• el-oued region
• flavonoid
• frap
• polyphenol
• solanum nigrum l.
• stem