A transportome-scale amiRNA-based screen identifies redundant roles of Arabidopsis ABCB6 and ABCB20 in auxin transport

Yuqin Zhang; Victoria Nasser; Odelia Pisanty; Moutasem Omary; Nikolai Wulff; Martin Di Donato; Iris Tal; Felix Hauser; Pengchao Hao; Ohad Roth; Hillel Fromm; Julian I. Schroeder; Markus Geisler; Hussam Hassan Nour-Eldin; Eilon Shani

doi:10.1038/s41467-018-06410-y

Nature Communications (Oct 2018)

A transportome-scale amiRNA-based screen identifies redundant roles of Arabidopsis ABCB6 and ABCB20 in auxin transport

Yuqin Zhang,
Victoria Nasser,
Odelia Pisanty,
Moutasem Omary,
Nikolai Wulff,
Martin Di Donato,
Iris Tal,
Felix Hauser,
Pengchao Hao,
Ohad Roth,
Hillel Fromm,
Julian I. Schroeder,
Markus Geisler,
Hussam Hassan Nour-Eldin,
Eilon Shani

Affiliations

Yuqin Zhang: School of Plant Sciences and Food Security, Tel Aviv University
Victoria Nasser: School of Plant Sciences and Food Security, Tel Aviv University
Odelia Pisanty: School of Plant Sciences and Food Security, Tel Aviv University
Moutasem Omary: School of Plant Sciences and Food Security, Tel Aviv University
Nikolai Wulff: DynaMo Center, Copenhagen Plant Science Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen
Martin Di Donato: Department of Biology, University of Fribourg
Iris Tal: School of Plant Sciences and Food Security, Tel Aviv University
Felix Hauser: Division of Biological Sciences, Cell and Developmental Biology Section, University of California, San Diego
Pengchao Hao: Department of Biology, University of Fribourg
Ohad Roth: School of Plant Sciences and Food Security, Tel Aviv University
Hillel Fromm: School of Plant Sciences and Food Security, Tel Aviv University
Julian I. Schroeder: Division of Biological Sciences, Cell and Developmental Biology Section, University of California, San Diego
Markus Geisler: Department of Biology, University of Fribourg
Hussam Hassan Nour-Eldin: DynaMo Center, Copenhagen Plant Science Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen
Eilon Shani: School of Plant Sciences and Food Security, Tel Aviv University

DOI: https://doi.org/10.1038/s41467-018-06410-y
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 12

Abstract

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Characterizing plant membrane transporters via genetic methods is complicated by functional redundancy among multi-gene transporter families. Here Zhang et al. use an artificial microRNA-based screen to overcome this issue and show that ABCB6 and ABCB20 act redundantly to regulate auxin transport.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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