Infection and Drug Resistance (Nov 2022)
Detection of Antibiotic Resistance Genes in Pseudomonas aeruginosa by Whole Genome Sequencing
Abstract
Omar B Ahmed Department of Environmental and Health Research, The Custodian of the Two Holy Mosques Institute of Hajj and Umrah Research, Umm Al-Qura University, Makkah, Saudi ArabiaCorrespondence: Omar B Ahmed, Email [email protected]: Multidrug-resistant Pseudomonas aeruginosa has become a hazard to public health, making medical treatment challenging and ineffective. Whole-genome sequencing for antibiotic susceptibility testing offers a powerful replacement for conventional microbiological methods.Objective: The present study evaluated the presence of antibiotic resistance genes in selected clinical strains of P. aeruginosa using whole-genome sequencing for antibiotic susceptibility testing.Results: Whole-genome sequencing of P. aeruginosa susceptible to common antibiotics showed the presence of 4 antibiotic resistance gene types, fosA, catB7, blaPAO, and blaOXA-50. Whole genome sequencing of resistant or multidrug-resistant P. aeruginosa showed the presence of multiple ARGs, such as sul1, aac(3)-Ic, blaPAO, blaGES-1, blaGES-5 aph (3’)-XV, blaOXA-50, aacA4, catB7, aph(3’)-IIb, aadA6, fosA, tet(G), cmlA1, aac(6’)Ib-cr, and rmtF.Conclusion: The acquisition of antibiotic resistance genes was found to depend on the resistance of Pseudomonas to antibiotics. The strain with the highest resistance to antibiotics had the highest acquisition of antibiotic resistance genes. MDR-P. aeruginosa produces antibiotic resistance genes against aminoglycoside, β-lactam, fluoroquinolones, sulfonamides, phenicol, and fosfomycin antibiotics.Keywords: antibiotic resistance, genes, Pseudomonas aeruginosa, whole genome, sequencing
