The Dıagnosıs of Human Leukocyte Antıgen Class I and Class II Allel ın Eastern Anatolıa Regıon

Abstract

INTRODUCTION: The aim of our study was to investigate the frequency of HLA class I and class II alleles in Eastern Anatolia. METHODS: The study included 1050 unrelated bone marrow donors from the archives. HLA class I and II polymorphisms were identified using polymerase chain reactions (PCR) using sequence-specific primers (SSP) and sequence-specific oligonucleotides (SSO). Ethical approval was obtained. RESULTS: The most common alleles were HLA-A*02 (21.1%), HLA-A*24 (18.4%), and HLA-A*03 (12.3%) in the HLA-A locus; HLA-B*35 (20%), HLA-B*51 (18.3%), and HLA-B*44 (6.3%) in the HLA-B locus; and HLA-DRB1*11 (22.3%), HLA-DRB1*04 (16.2%), and HLA-DRB1*15 (12.4%) in the HLA-DR locus. DISCUSSION AND CONCLUSION: : Our results are consistent with the HLA antigens identified in previous studies. We believe that our study will contribute to the determination of HLA diversity in our region.. Consolidating the data obtained in this study and other national data will facilitate the selection of bone marrow donors

Keywords

• hla
• class i
• class ii