Frontiers in Physics (May 2022)

High Spatio-Temporal Resolution Condenser-Free Quantitative Phase Contrast Microscopy

  • Ying Ma,
  • Ying Ma,
  • Lin Ma,
  • Juanjuan Zheng,
  • Juanjuan Zheng,
  • Min Liu,
  • Zeev Zalevsky,
  • Peng Gao

DOI
https://doi.org/10.3389/fphy.2022.892529
Journal volume & issue
Vol. 10

Abstract

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Quantitative phase-contrast microscopy (QPCM) provides an effective approach for label-free detection of transparent samples. In this study, we propose a condenser-free quantitative phase-contrast microscopy (CF-QPCM), in which several light-emitting diodes (LEDs) distributed on a ring are used for direct ultra-oblique illumination. Such condenser-free design greatly simplifies the system’s structure and releases the space for installing samples. Quantitative phase maps are reconstructed by retarding the unscattered components of the object waves for a series of phases 0, π/2, π, and 3π/2 through a high-speed spatial light modulator (SLM). With this system, quantitative phase imaging of live cells has been achieved at a spatial resolution of 231 nm (lateral) and a frame rate of 250 Hz. We believe that the proposed CF-QPCM can contribute to biomedical, industrial, chemistry fields, etc.

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