Extraction, Structural Characterization, and Anti-Hepatocellular Carcinoma Activity of Polysaccharides From Panax ginseng Meyer

Hui Jia; Bin Zhao; Fangfang Zhang; Ramesh Kumar Santhanam; Xinying Wang; Jincai Lu; Jincai Lu

doi:10.3389/fonc.2021.785455

Frontiers in Oncology (Nov 2021)

Extraction, Structural Characterization, and Anti-Hepatocellular Carcinoma Activity of Polysaccharides From Panax ginseng Meyer

Hui Jia,
Bin Zhao,
Fangfang Zhang,
Ramesh Kumar Santhanam,
Xinying Wang,
Jincai Lu,
Jincai Lu

Affiliations

Hui Jia: School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, China
Bin Zhao: School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, China
Fangfang Zhang: Department of Stomatology, The Fifth Medical Center of People’s Liberation Army (PLA) General Hospital, Beijing, China
Ramesh Kumar Santhanam: Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, Kuala Nerus, Malaysia
Xinying Wang: School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, China
Jincai Lu: School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, China
Jincai Lu: Liaoning Provincial Key Laboratory of Traditional Chinese Medicine (TCM) Resources Conservation and Development, Shenyang Pharmaceutical University, Shenyang, China

DOI: https://doi.org/10.3389/fonc.2021.785455
Journal volume & issue: Vol. 11

Abstract

Read online

Polysaccharides are the main active ingredients of ginseng. To extract the most effective polysaccharides against hepatocellular carcinoma (HCC), we isolated and characterized the polysaccharides from the mountain cultivated ginseng (MCG) and compared their composition and cytotoxic effect with cultivated ginseng (CG) polysaccharide against HepG2 cell lines for the first time. MCG polysaccharides and CG polysaccharides were fractionated into two fractions such as MTPS-1, MTPS-2 and CTPS-1, CTPS-2 by salting out, respectively. Compared to CG, MCG possessed appreciable cytotoxic effect against HepG2 cells among that MTPS-1 possess fortified effect. Then, MTPS-1 was selected for further isolation process and seven acidic polysaccharides (MCGP-1–MCGP-7) were obtained using ethanol precipitation, ion-exchange, and gel permeation chromatography techniques. Structural characteristics of the polysaccharides (MCGP-1–MCGP-7) were done by adapting methylation/GC-MS and NMR analysis. Overall, MCGP-3 polysaccharide was found to possess significant cytotoxic effect against HepG2 cells with the IC50 value.

Published in Frontiers in Oncology

ISSN: 2234-943X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Internal medicine: Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Website: https://www.frontiersin.org/journals/oncology/

About the journal

Abstract

Keywords