Journal of International Medical Research (Nov 2021)

Docking protein 1 and free fatty acids are associated with insulin resistance in patients with type 2 diabetes mellitus

  • Xiaoqin Ha,
  • Xiaoling Cai,
  • Huizhe Cao,
  • Jie Li,
  • Bo Yang,
  • Ruru Jiang,
  • Xin Li,
  • Bin Li,
  • Yuan Xin

DOI
https://doi.org/10.1177/03000605211048293
Journal volume & issue
Vol. 49

Abstract

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Objective Insulin resistance (IR) is a key defect in type 2 diabetes mellitus (T2DM); therefore, effective means of ameliorating IR are sought. Methods We performed a retrospective cohort study of 154 patients with T2DM and 39 with pre-diabetes (pre-DM). The effects of IR and a high concentration of FFA on gene expression were determined using microarray analysis and quantitative reverse transcription polymerase chain reaction (RT-qPCR) in patients with T2DM or pre-DM. Results Serum FFA concentration and homeostasis model assessment of IR (HOMA-IR) were significantly higher in patients with T2DM but no obesity and in those with pre-DM than in controls. HOMA-IR was significantly associated with T2DM. RT-qPCR showed that the expression of FBJ murine osteosarcoma viral oncogene homolog ( FOS ) and AE binding protein 1 ( AEBP1 ) was much lower in the circulation of participants with obesity and diabetes. RT-qPCR showed that the expression of docking protein 1 ( DOK1 ) was significantly lower in the blood of participants with diabetes but no obesity and in those with pre-DM than in controls. Conclusions FFA and DOK1 are associated with IR in patients with T2DM but no obesity or pre-DM. The downregulation of DOK1 might inhibit lipid synthesis and induce lipolysis, inducing or worsening IR.