Sensor-regulator and RNAi based bifunctional dynamic control network for engineered microbial synthesis

Yaping Yang; Yuheng Lin; Jian Wang; Yifei Wu; Ruihua Zhang; Mengyin Cheng; Xiaolin Shen; Jia Wang; Zhenya Chen; Chenyi Li; Qipeng Yuan; Yajun Yan

doi:10.1038/s41467-018-05466-0

Nature Communications (Aug 2018)

Sensor-regulator and RNAi based bifunctional dynamic control network for engineered microbial synthesis

Yaping Yang,
Yuheng Lin,
Jian Wang,
Yifei Wu,
Ruihua Zhang,
Mengyin Cheng,
Xiaolin Shen,
Jia Wang,
Zhenya Chen,
Chenyi Li,
Qipeng Yuan,
Yajun Yan

Affiliations

Yaping Yang: School of Chemical, Materials and Biomedical Engineering, College of Engineering, The University of Georgia
Yuheng Lin: School of Chemical, Materials and Biomedical Engineering, College of Engineering, The University of Georgia
Jian Wang: School of Chemical, Materials and Biomedical Engineering, College of Engineering, The University of Georgia
Yifei Wu: Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology
Ruihua Zhang: School of Chemical, Materials and Biomedical Engineering, College of Engineering, The University of Georgia
Mengyin Cheng: School of Chemical, Materials and Biomedical Engineering, College of Engineering, The University of Georgia
Xiaolin Shen: Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology
Jia Wang: Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology
Zhenya Chen: Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology
Chenyi Li: School of Chemical, Materials and Biomedical Engineering, College of Engineering, The University of Georgia
Qipeng Yuan: Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology
Yajun Yan: School of Chemical, Materials and Biomedical Engineering, College of Engineering, The University of Georgia

DOI: https://doi.org/10.1038/s41467-018-05466-0
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 10

Abstract

Read online

Engineering dynamic control can improve microbial production of target chemicals. Here, the authors design a sensor-regulator and RNAi based bifunctional dynamic control network that can simultaneously and independently turn up and down cellular metabolism for engineered muconic acid production in E. coli.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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