Enzymatic and ultrasound assisted β-cyclodextrin extraction of active ingredients from Forsythia suspensa and their antioxidant and anti-inflammatory activities

Xiaoyue Xiao; Yang Zhang; Kedi Sun; Shuoqi Liu; Qingmiao Li; Yu Zhang; Bello-Onaghise Godspower; Tong Xu; Zhiyun Zhang; Yanhua Li; Yanyan Liu

Ultrasonics Sonochemistry (Aug 2024)

Enzymatic and ultrasound assisted β-cyclodextrin extraction of active ingredients from Forsythia suspensa and their antioxidant and anti-inflammatory activities

Xiaoyue Xiao,
Yang Zhang,
Kedi Sun,
Shuoqi Liu,
Qingmiao Li,
Yu Zhang,
Bello-Onaghise Godspower,
Tong Xu,
Zhiyun Zhang,
Yanhua Li,
Yanyan Liu

Affiliations

Xiaoyue Xiao: College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, China; Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, China
Yang Zhang: College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, China; Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, China
Kedi Sun: College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, China; Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, China
Shuoqi Liu: College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, China; Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, China
Qingmiao Li: College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, China; Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, China
Yu Zhang: College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, China; Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, China
Bello-Onaghise Godspower: College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, China; Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, China; Department of Animal Science, Faculty of Agriculture, University of Benin City, Nigeria
Tong Xu: College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, China; Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, China
Zhiyun Zhang: College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, China; Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, China
Yanhua Li: College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, China; Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, China; Corresponding authors at: College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
Yanyan Liu: College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, China; Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, China; Corresponding authors at: College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.

Journal volume & issue: Vol. 108
p. 106944

Abstract

Read online

With the proposal of the 2030 Agenda for Sustainable Development, the Chinese medicine extraction technology has been innovatively improved to prioritize low energy consumption, sustainability, and minimized organic solvent utilization. Forsythia suspensa (FS) possesses favorable pharmacological properties and is extensively utilized in traditional Chinese medicine. However, due to the limitations of the composition and extraction methods, its potential has not been fully developed. Thus, a combination of ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), and β-cyclodextrin extraction (β-CDE) was employed to isolate and purify rutin, phillyrin, and forsythoside A from FS. The results demonstrated that the efficiency of extracting enzymatic and ultrasound assisted β-cyclodextrin extraction (EUA-β-CDE) was highly influenced by the temperature and duration of hydrolysis, as well as the duration of the extraction process. According to the results of the single-factor experiment, Box-Behnken design (BBD) in Response surface method (RSM) was used to optimize the experimental parameters to achieve the maximum comprehensive evaluation value (CEV) value. The EUA-β-CDE compared with other extraction methods, has good extraction effect and low energy consumption by high performance liquid chromatography (HPLC), scanning electron microscopy (SEM), calculation of power consumption and CO2 emission The EUA-β-CDE compared with other extraction methods, has good extraction effect and low energy consumption by HPLC, SEM, calculation of power consumption and CO2 emission. Then, the structural characteristics of EUA-β-CDE of FS extract had significant interaction with β-CD by Fourier infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC). In addition, EUA-β-CDE extract has good antioxidant and anti-inflammatory activities. The establishment of EUA-β-CDE of FS provides a new idea for the development and application of other sustainable extraction methods of traditional Chinese medicine.

Published in Ultrasonics Sonochemistry

ISSN: 1350-4177 (Print); 1873-2828 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Chemistry; Science: Physics: Acoustics. Sound
Website: https://www.journals.elsevier.com/ultrasonics-sonochemistry

About the journal

Abstract

Keywords