iScience (Jan 2023)
Sex-inducing effects toward planarians widely present among parasitic flatworms
- Kiyono Sekii,
- Soichiro Miyashita,
- Kentaro Yamaguchi,
- Ikuma Saito,
- Yuria Saito,
- Sayaka Manta,
- Masaki Ishikawa,
- Miyu Narita,
- Taro Watanabe,
- Riku Ito,
- Mizuki Taguchi,
- Ryohei Furukawa,
- Aoi Ikeuchi,
- Kayoko Matsuo,
- Goro Kurita,
- Takashi Kumagai,
- Sho Shirakashi,
- Kazuo Ogawa,
- Kimitoshi Sakamoto,
- Ryo Koyanagi,
- Noriyuki Satoh,
- Mizuki Sasaki,
- Takanobu Maezawa,
- Madoka Ichikawa-Seki,
- Kazuya Kobayashi
Affiliations
- Kiyono Sekii
- Department of Biology, Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki, Aomori 036-8561, Japan; Corresponding author
- Soichiro Miyashita
- Department of Biology, Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki, Aomori 036-8561, Japan
- Kentaro Yamaguchi
- Department of Biology, Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki, Aomori 036-8561, Japan
- Ikuma Saito
- Department of Biology, Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki, Aomori 036-8561, Japan
- Yuria Saito
- Department of Biology, Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki, Aomori 036-8561, Japan
- Sayaka Manta
- Department of Biology, Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki, Aomori 036-8561, Japan
- Masaki Ishikawa
- Department of Biology, Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki, Aomori 036-8561, Japan
- Miyu Narita
- Department of Biology, Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki, Aomori 036-8561, Japan
- Taro Watanabe
- Department of Biology, Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki, Aomori 036-8561, Japan
- Riku Ito
- Department of Biology, Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki, Aomori 036-8561, Japan
- Mizuki Taguchi
- Department of Biology, Research and Education Center for Natural Sciences, Keio University, 4-1-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223-8521, Japan
- Ryohei Furukawa
- Department of Biology, Research and Education Center for Natural Sciences, Keio University, 4-1-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223-8521, Japan
- Aoi Ikeuchi
- Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan
- Kayoko Matsuo
- Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan
- Goro Kurita
- Kurita Animal Hospital, 139-1 Koga, Koga, Ibaraki 306-0016, Japan
- Takashi Kumagai
- Section of Environmental Parasitology, Graduate School, Tokyo Medical & Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8510, Japan
- Sho Shirakashi
- Aquaculture Research Institute, Kindai University, Shirahama 3153, Nishimuro-gun, Wakayama 649-2211, Japan
- Kazuo Ogawa
- Meguro Parasitological Museum, 4-1-1 Shimo-Meguro, Meguro, Tokyo 153-0064, Japan
- Kimitoshi Sakamoto
- Department of Biology, Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki, Aomori 036-8561, Japan
- Ryo Koyanagi
- Marine Genomics Unit, Okinawa Institute of Science and Technology Graduate University, Onna, Okinawa 904-0495, Japan
- Noriyuki Satoh
- Marine Genomics Unit, Okinawa Institute of Science and Technology Graduate University, Onna, Okinawa 904-0495, Japan
- Mizuki Sasaki
- Department of Parasitology, Asahikawa Medical University, 2-1-1-1 Midorigaoka-Higashi, Asahikawa, Hokkaido 078-8510, Japan
- Takanobu Maezawa
- Department of Integrated Science and Technology, National Institute of Technology, Tsuyama College, 624-1 Numa, Tsuyama, Okayama 708–8509, Japan
- Madoka Ichikawa-Seki
- Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan; Corresponding author
- Kazuya Kobayashi
- Department of Biology, Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki, Aomori 036-8561, Japan; Corresponding author
- Journal volume & issue
-
Vol. 26,
no. 1
p. 105776
Abstract
Summary: Various parasitic flatworms infect vertebrates for sexual reproduction, often causing devastating diseases in their hosts. Consequently, flatworms are of great socioeconomic and biomedical importance. Although the cessation of parasitic flatworm sexual reproduction is a major target of anti-parasitic drug design, little is known regarding bioactive compounds controlling flatworm sexual maturation. Using the planarian Dugesia ryukyuensis, we observed that sex-inducing substances found in planarians are also widespread in parasitic flatworms, such as monogeneans and flukes (but not in tapeworms). Reverse-phase HPLC analysis revealed the sex-inducing substance(s) eluting around the tryptophan retention time in the fluke Calicophoron calicophorum, consistent with previous studies on the planarian Bipalium nobile, suggesting that the substance(s) is likely conserved among flatworms. Moreover, six of the 18 ovary-inducing substances identified via transcriptome and metabolome analyses are involved in purine metabolism. Our findings provide a basis for understanding and modifying the life cycles of various parasitic flatworms.
