The Inhibitory Effect of Ileal Mucosal Media Originated from FVB/N Mice Strain on Escherichia coli LF82 Invasion

Abstract

Objective:The aim of this study was to investigate the effect of healthy mucosa on adhesive and invasive properties of AIEC reference strain Escherichia coli (E. coli) LF82. For this purpose, we had designed special medias that contained cell culture medium and mucosal content obtained from different regions (colon and ileum) of the digestive tract.Methods:We tested the infecting ability of AIEC reference strain E. coli LF82 on I-407 cells in the presence of mucosal media (Muc-M) under in vitro conditions. Muc-M composed of certain rates of cell culture medium or M63 minimal medium and mucosal contents obtained from different part of intestine were designed for cellinfection experiments and biofilm-formation assays.Results:The result showed that the mucosal media decreased the infection percentage of E. coli LF82 strain when compared with control group. It was seen that the mucosal media originating from ileum almost completely inhibited the invasion of LF82 strain. On the other hand, it was observed that the mucosal media prepared from colon) reduced the bacterial invasion only in half the rate when compared with control.Conclusion:The findings showed that these medias obtained from different regions of the intestinal tract affected LF82 invasion at different rates. Therefore, this study provided crucial information that could contribute to the future studies on the localization of bacteria.

Keywords

• Escherichia coli LF82
• Crohn’s disease
• biofilm
• pathobiont
• adhesion
• invasion