Direct quality control of glycoengineered erythropoietin variants

Tomislav Čaval; Weihua Tian; Zhang Yang; Henrik Clausen; Albert J. R. Heck

doi:10.1038/s41467-018-05536-3

Nature Communications (Aug 2018)

Direct quality control of glycoengineered erythropoietin variants

Tomislav Čaval,
Weihua Tian,
Zhang Yang,
Henrik Clausen,
Albert J. R. Heck

Affiliations

Tomislav Čaval: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Science4Life, University of Utrecht
Weihua Tian: Copenhagen Center for Glycomics, Department of Cellular and Molecular Medicine, Faculty of Health Sciences, University of Copenhagen
Zhang Yang: Copenhagen Center for Glycomics, Department of Cellular and Molecular Medicine, Faculty of Health Sciences, University of Copenhagen
Henrik Clausen: Copenhagen Center for Glycomics, Department of Cellular and Molecular Medicine, Faculty of Health Sciences, University of Copenhagen
Albert J. R. Heck: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Science4Life, University of Utrecht

DOI: https://doi.org/10.1038/s41467-018-05536-3
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 8

Abstract

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Several therapeutics are glycosylated proteins, yet the analysis of their specific glycosylation patterns remains challenging. Here the authors demonstrate an approach for the detailed characterization of glycosylated biopharmaceuticals applied to the determination of the glycoproteoform profile of glycoengineered variants of erythropoietin.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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