A Temporal Proteomic Map of Epstein-Barr Virus Lytic Replication in B Cells

Ina Ersing; Luis Nobre; Liang Wei Wang; Lior Soday; Yijie Ma; Joao A. Paulo; Yohei Narita; Camille W. Ashbaugh; Chang Jiang; Nicholas E. Grayson; Elliott Kieff; Steven P. Gygi; Michael P. Weekes; Benjamin E. Gewurz

doi:10.1016/j.celrep.2017.04.062

Cell Reports (May 2017)

A Temporal Proteomic Map of Epstein-Barr Virus Lytic Replication in B Cells

Ina Ersing,
Luis Nobre,
Liang Wei Wang,
Lior Soday,
Yijie Ma,
Joao A. Paulo,
Yohei Narita,
Camille W. Ashbaugh,
Chang Jiang,
Nicholas E. Grayson,
Elliott Kieff,
Steven P. Gygi,
Michael P. Weekes,
Benjamin E. Gewurz

Affiliations

Ina Ersing: Division of Infectious Disease, Department of Medicine, Brigham & Women’s Hospital, Harvard Medical School, 181 Longwood Avenue, Boston, MA 02115, USA
Luis Nobre: Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK
Liang Wei Wang: Division of Infectious Disease, Department of Medicine, Brigham & Women’s Hospital, Harvard Medical School, 181 Longwood Avenue, Boston, MA 02115, USA
Lior Soday: Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK
Yijie Ma: Division of Infectious Disease, Department of Medicine, Brigham & Women’s Hospital, Harvard Medical School, 181 Longwood Avenue, Boston, MA 02115, USA
Joao A. Paulo: Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
Yohei Narita: Division of Infectious Disease, Department of Medicine, Brigham & Women’s Hospital, Harvard Medical School, 181 Longwood Avenue, Boston, MA 02115, USA
Camille W. Ashbaugh: Division of Infectious Disease, Department of Medicine, Brigham & Women’s Hospital, Harvard Medical School, 181 Longwood Avenue, Boston, MA 02115, USA
Chang Jiang: Division of Infectious Disease, Department of Medicine, Brigham & Women’s Hospital, Harvard Medical School, 181 Longwood Avenue, Boston, MA 02115, USA
Nicholas E. Grayson: Wellcome Trust Sanger Institute, Hinxton, Cambridge, CB10 1HH, UK
Elliott Kieff: Division of Infectious Disease, Department of Medicine, Brigham & Women’s Hospital, Harvard Medical School, 181 Longwood Avenue, Boston, MA 02115, USA
Steven P. Gygi: Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
Michael P. Weekes: Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK
Benjamin E. Gewurz: Division of Infectious Disease, Department of Medicine, Brigham & Women’s Hospital, Harvard Medical School, 181 Longwood Avenue, Boston, MA 02115, USA

DOI: https://doi.org/10.1016/j.celrep.2017.04.062
Journal volume & issue: Vol. 19, no. 7
pp. 1479 – 1493

Abstract

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Epstein-Barr virus (EBV) replication contributes to multiple human diseases, including infectious mononucleosis, nasopharyngeal carcinoma, B cell lymphomas, and oral hairy leukoplakia. We performed systematic quantitative analyses of temporal changes in host and EBV proteins during lytic replication to gain insights into virus-host interactions, using conditional Burkitt lymphoma models of type I and II EBV infection. We quantified profiles of >8,000 cellular and 69 EBV proteins, including >500 plasma membrane proteins, providing temporal views of the lytic B cell proteome and EBV virome. Our approach revealed EBV-induced remodeling of cell cycle, innate and adaptive immune pathways, including upregulation of the complement cascade and proteasomal degradation of the B cell receptor complex, conserved between EBV types I and II. Cross-comparison with proteomic analyses of human cytomegalovirus infection and of a Kaposi-sarcoma-associated herpesvirus immunoevasin identified host factors targeted by multiple herpesviruses. Our results provide an important resource for studies of EBV replication.

Published in Cell Reports

ISSN: 2211-1247 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: http://www.cell.com/cell-reports/home

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