Isobutanol production freed from biological limits using synthetic biochemistry

Saken Sherkhanov; Tyler P. Korman; Sum Chan; Salem Faham; Hongjiang Liu; Michael R. Sawaya; Wan-Ting Hsu; Ellee Vikram; Tiffany Cheng; James U. Bowie

doi:10.1038/s41467-020-18124-1

Nature Communications (Aug 2020)

Isobutanol production freed from biological limits using synthetic biochemistry

Saken Sherkhanov,
Tyler P. Korman,
Sum Chan,
Salem Faham,
Hongjiang Liu,
Michael R. Sawaya,
Wan-Ting Hsu,
Ellee Vikram,
Tiffany Cheng,
James U. Bowie

Affiliations

Saken Sherkhanov: Department of Chemistry and Biochemistry, Molecular Biology Institute, UCLA-DOE Institute, University of California
Tyler P. Korman: Department of Chemistry and Biochemistry, Molecular Biology Institute, UCLA-DOE Institute, University of California
Sum Chan: Department of Chemistry and Biochemistry, Molecular Biology Institute, UCLA-DOE Institute, University of California
Salem Faham: Vertex Pharmaceuticals
Hongjiang Liu: Department of Chemistry and Biochemistry, Molecular Biology Institute, UCLA-DOE Institute, University of California
Michael R. Sawaya: Department of Chemistry and Biochemistry, Molecular Biology Institute, UCLA-DOE Institute, University of California
Wan-Ting Hsu: Department of Chemistry and Biochemistry, Molecular Biology Institute, UCLA-DOE Institute, University of California
Ellee Vikram: Department of Chemistry and Biochemistry, Molecular Biology Institute, UCLA-DOE Institute, University of California
Tiffany Cheng: Department of Chemistry and Biochemistry, Molecular Biology Institute, UCLA-DOE Institute, University of California
James U. Bowie: Department of Chemistry and Biochemistry, Molecular Biology Institute, UCLA-DOE Institute, University of California

DOI: https://doi.org/10.1038/s41467-020-18124-1
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 10

Abstract

Read online

A cell free or synthetic biochemistry approach offers a way to circumvent the many constraints of living cells. Here, the authors demonstrate, via enzyme and process enhancements, the production of isobutanol with the metrics exceeding highly developed ethanol fermentation.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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