Nature Communications (Oct 2021)
High-throughput and high-efficiency sample preparation for single-cell proteomics using a nested nanowell chip
- Jongmin Woo,
- Sarah M. Williams,
- Lye Meng Markillie,
- Song Feng,
- Chai-Feng Tsai,
- Victor Aguilera-Vazquez,
- Ryan L. Sontag,
- Ronald J. Moore,
- Dehong Hu,
- Hardeep S. Mehta,
- Joshua Cantlon-Bruce,
- Tao Liu,
- Joshua N. Adkins,
- Richard D. Smith,
- Geremy C. Clair,
- Ljiljana Pasa-Tolic,
- Ying Zhu
Affiliations
- Jongmin Woo
- Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory
- Sarah M. Williams
- Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory
- Lye Meng Markillie
- Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory
- Song Feng
- Biological Sciences Division, Pacific Northwest National Laboratory
- Chai-Feng Tsai
- Biological Sciences Division, Pacific Northwest National Laboratory
- Victor Aguilera-Vazquez
- Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory
- Ryan L. Sontag
- Biological Sciences Division, Pacific Northwest National Laboratory
- Ronald J. Moore
- Biological Sciences Division, Pacific Northwest National Laboratory
- Dehong Hu
- Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory
- Hardeep S. Mehta
- Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory
- Joshua Cantlon-Bruce
- Scienion AG
- Tao Liu
- Biological Sciences Division, Pacific Northwest National Laboratory
- Joshua N. Adkins
- Biological Sciences Division, Pacific Northwest National Laboratory
- Richard D. Smith
- Biological Sciences Division, Pacific Northwest National Laboratory
- Geremy C. Clair
- Biological Sciences Division, Pacific Northwest National Laboratory
- Ljiljana Pasa-Tolic
- Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory
- Ying Zhu
- Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory
- DOI
- https://doi.org/10.1038/s41467-021-26514-2
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 11
Abstract
Single-cell proteomics is an emerging technology but protein coverage, throughput and quantitation accuracy are often still insufficient. Here, the authors develop a nested nanowell chip that improves protein recovery, throughput and robustness of isobaric labeling-based quantitative single-cell proteomics.
