Engineered helicase replaces thermocycler in DNA amplification while retaining desired PCR characteristics

Momčilo Gavrilov; Joshua Y. C. Yang; Roger S. Zou; Wen Ma; Chun-Ying Lee; Sonisilpa Mohapatra; Jimin Kang; Ting-Wei Liao; Sua Myong; Taekjip Ha

doi:10.1038/s41467-022-34076-0

Nature Communications (Oct 2022)

Engineered helicase replaces thermocycler in DNA amplification while retaining desired PCR characteristics

Momčilo Gavrilov,
Joshua Y. C. Yang,
Roger S. Zou,
Wen Ma,
Chun-Ying Lee,
Sonisilpa Mohapatra,
Jimin Kang,
Ting-Wei Liao,
Sua Myong,
Taekjip Ha

Affiliations

Momčilo Gavrilov: Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine
Joshua Y. C. Yang: Department of Biomedical Engineering, Johns Hopkins University School of Medicine
Roger S. Zou: Department of Biomedical Engineering, Johns Hopkins University School of Medicine
Wen Ma: Department of Chemistry and Biochemistry, University of California
Chun-Ying Lee: Department of Biophysics, Johns Hopkins University
Sonisilpa Mohapatra: Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine
Jimin Kang: Department of Biophysics, Johns Hopkins University
Ting-Wei Liao: Department of Biophysics, Johns Hopkins University
Sua Myong: Department of Biophysics, Johns Hopkins University
Taekjip Ha: Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine

DOI: https://doi.org/10.1038/s41467-022-34076-0
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 14

Abstract

Read online

PCR is an essential method for the amplification and manipulation of nucleic acids, but the requirement for a thermocycler limits access. Here, authors engineer a helicase to replace the heating step of PCR with enzymatic unwinding, allowing the isothermal amplification of fragments up to 6 kb.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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