Frontiers in Cell and Developmental Biology (May 2024)
Digital droplet PCR analysis of organoids generated from mouse mammary tumors demonstrates proof-of-concept capture of tumor heterogeneity
- Katherine E. Lake,
- Katherine E. Lake,
- Megan M. Colonnetta,
- Megan M. Colonnetta,
- Megan M. Colonnetta,
- Megan M. Colonnetta,
- Clayton A. Smith,
- Clayton A. Smith,
- Kaitlyn Saunders,
- Kaitlyn Saunders,
- Kenneth Martinez-Algarin,
- Kenneth Martinez-Algarin,
- Sakshi Mohta,
- Sakshi Mohta,
- Jacob Pena,
- Jacob Pena,
- Heather L. McArthur,
- Heather L. McArthur,
- Sangeetha M. Reddy,
- Sangeetha M. Reddy,
- Evanthia T. Roussos Torres,
- Elizabeth H. Chen,
- Elizabeth H. Chen,
- Elizabeth H. Chen,
- Elizabeth H. Chen,
- Isaac S. Chan,
- Isaac S. Chan,
- Isaac S. Chan,
- Isaac S. Chan
Affiliations
- Katherine E. Lake
- Department of Internal Medicine, Division of Hematology and Oncology, University of Texas Southwestern, Dallas, TX, United States
- Katherine E. Lake
- Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX, United States
- Megan M. Colonnetta
- Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX, United States
- Megan M. Colonnetta
- Department of Molecular Biology, University of Texas Southwestern, Dallas, TX, United States
- Megan M. Colonnetta
- Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX, United States
- Megan M. Colonnetta
- Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX, United States
- Clayton A. Smith
- Department of Internal Medicine, Division of Hematology and Oncology, University of Texas Southwestern, Dallas, TX, United States
- Clayton A. Smith
- Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX, United States
- Kaitlyn Saunders
- Department of Internal Medicine, Division of Hematology and Oncology, University of Texas Southwestern, Dallas, TX, United States
- Kaitlyn Saunders
- Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX, United States
- Kenneth Martinez-Algarin
- Department of Internal Medicine, Division of Hematology and Oncology, University of Texas Southwestern, Dallas, TX, United States
- Kenneth Martinez-Algarin
- Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX, United States
- Sakshi Mohta
- Department of Internal Medicine, Division of Hematology and Oncology, University of Texas Southwestern, Dallas, TX, United States
- Sakshi Mohta
- Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX, United States
- Jacob Pena
- Department of Internal Medicine, Division of Hematology and Oncology, University of Texas Southwestern, Dallas, TX, United States
- Jacob Pena
- Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX, United States
- Heather L. McArthur
- Department of Internal Medicine, Division of Hematology and Oncology, University of Texas Southwestern, Dallas, TX, United States
- Heather L. McArthur
- Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX, United States
- Sangeetha M. Reddy
- Department of Internal Medicine, Division of Hematology and Oncology, University of Texas Southwestern, Dallas, TX, United States
- Sangeetha M. Reddy
- Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX, United States
- Evanthia T. Roussos Torres
- Division of Medical Oncology, Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, CA, United States
- Elizabeth H. Chen
- Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX, United States
- Elizabeth H. Chen
- Department of Molecular Biology, University of Texas Southwestern, Dallas, TX, United States
- Elizabeth H. Chen
- Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX, United States
- Elizabeth H. Chen
- Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX, United States
- Isaac S. Chan
- Department of Internal Medicine, Division of Hematology and Oncology, University of Texas Southwestern, Dallas, TX, United States
- Isaac S. Chan
- Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX, United States
- Isaac S. Chan
- Department of Molecular Biology, University of Texas Southwestern, Dallas, TX, United States
- Isaac S. Chan
- Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX, United States
- DOI
- https://doi.org/10.3389/fcell.2024.1358583
- Journal volume & issue
-
Vol. 12
Abstract
Breast cancer metastases exhibit many different genetic alterations, including copy number amplifications (CNA). CNA are genetic alterations that are increasingly becoming relevant to breast oncology clinical practice. Here we identify CNA in metastatic breast tumor samples using publicly available datasets and characterize their expression and function using a metastatic mouse model of breast cancer. Our findings demonstrate that our organoid generation can be implemented to study clinically relevant features that reflect the genetic heterogeneity of individual tumors.
Keywords
