Nature Communications (Apr 2018)

True equilibrium measurement of transcription factor-DNA binding affinities using automated polarization microscopy

  • Christophe Jung,
  • Peter Bandilla,
  • Marc von Reutern,
  • Max Schnepf,
  • Susanne Rieder,
  • Ulrich Unnerstall,
  • Ulrike Gaul

DOI
https://doi.org/10.1038/s41467-018-03977-4
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 11

Abstract

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Methods to measure selective transcription factor-DNA binding often lack sensitivity and are not performed in solution. Here the authors develop a method to perform fluorescence anisotropy measurements of transcription factor-DNA binding energies with high sensitivity and throughput.