Nature Communications (May 2020)
mRNA display with library of even-distribution reveals cellular interactors of influenza virus NS1
- Yushen Du,
- Judd F. Hultquist,
- Quan Zhou,
- Anders Olson,
- Yenwen Tseng,
- Tian-hao Zhang,
- Mengying Hong,
- Kejun Tang,
- Liubo Chen,
- Xiangzhi Meng,
- Michael J. McGregor,
- Lei Dai,
- Danyang Gong,
- Laura Martin-Sancho,
- Sumit Chanda,
- Xinming Li,
- Steve Bensenger,
- Nevan J. Krogan,
- Ren Sun
Affiliations
- Yushen Du
- Department of Molecular and Medical Pharmacology, University of California
- Judd F. Hultquist
- Department of Cellular and Molecular Pharmacology, University of California, San Francisco
- Quan Zhou
- Department of Molecular and Medical Pharmacology, University of California
- Anders Olson
- Department of Molecular and Medical Pharmacology, University of California
- Yenwen Tseng
- Department of Molecular and Medical Pharmacology, University of California
- Tian-hao Zhang
- Department of Molecular and Medical Pharmacology, University of California
- Mengying Hong
- Cancer Institute, ZJU-UCLA Joint Center for Medical Education and Research, School of Medicine, Zhejiang University
- Kejun Tang
- Cancer Institute, ZJU-UCLA Joint Center for Medical Education and Research, School of Medicine, Zhejiang University
- Liubo Chen
- Cancer Institute, ZJU-UCLA Joint Center for Medical Education and Research, School of Medicine, Zhejiang University
- Xiangzhi Meng
- Department of Molecular and Medical Pharmacology, University of California
- Michael J. McGregor
- Department of Cellular and Molecular Pharmacology, University of California, San Francisco
- Lei Dai
- Department of Molecular and Medical Pharmacology, University of California
- Danyang Gong
- Department of Molecular and Medical Pharmacology, University of California
- Laura Martin-Sancho
- Sanford Burnham Prebys Medical Discovery Institute
- Sumit Chanda
- Sanford Burnham Prebys Medical Discovery Institute
- Xinming Li
- Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, L
- Steve Bensenger
- Department of Molecular and Medical Pharmacology, University of California
- Nevan J. Krogan
- Department of Cellular and Molecular Pharmacology, University of California, San Francisco
- Ren Sun
- Department of Molecular and Medical Pharmacology, University of California
- DOI
- https://doi.org/10.1038/s41467-020-16140-9
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 13
Abstract
Identification of low abundance proteins interacting with viral proteins can be challenging. Here, Du et al. develop an mRNA display approach with a library of even distribution, identify host proteins interacting with NS1 protein of influenza A virus, and show that one interactor provides a means to regulate cellular fatty acids synthesis.
