Mutation Analysis and Single Nucleotide Polymorphism (SNP) of TP53 in Breast CancerIn East Java

Abstract

The Incidence of Indonesia breast cancer case in2018 was reportedat 20.7% or160,653 in number. The factors that caused breast cancer is TP53gene mutationand Single Nucleotide Polymorphism (SNP). This study aimedto determine the mutation and Single Nucleotide Polymorphism (SNP) of TP53genein breast cancer. Samples ofthis study were 9 people chosen based on a purposive tech-nique. The methods include total DNA isolation, DNA quantification, PCR, and sequencing. The results of the sequencingwere then analyzed using alignment and blast. The SNP is browsedby the SNP finder on NCBI both followed by protein modeling. The results of this study indicate the existence of mutation in the exon and intron regions. Substitution of Guanine (G) to base Adenine (A)is occur in codon 496 which is a coding region. SNP was also obtained in exon of sample 12. The conclusion of this study is breast cancer can be caused by mutations that occur in the exonand altered the structur of protein structure, so then affect the binding affinity between p53 and its target domain.Tp53 gene mutation is not the only cause of breast cancer staging development, there are other genes that also con-tribute to the development of cancer stage.In addition, Single Nucleotide Poly-morphism in the TP53gene can be used as a predisposition marker of breast cancer that has potential to be inherited.

Keywords

• breast cancer
• mutation
• single nucleotide polymorphism
• tp53