Chitinolytic and Fungicidal Potential of the Marine Bacterial Strains Habituating Pacific Ocean Regions

Iuliia Pentekhina; Olga Nedashkovskaya; Aleksandra Seitkalieva; Vladimir Gorbach; Lubov Slepchenko; Natalya Kirichuk; Anna Podvolotskaya; Oksana Son; Liudmila Tekutyeva; Larissa Balabanova

doi:10.3390/microorganisms11092255

Microorganisms (Sep 2023)

Chitinolytic and Fungicidal Potential of the Marine Bacterial Strains Habituating Pacific Ocean Regions

Iuliia Pentekhina,
Olga Nedashkovskaya,
Aleksandra Seitkalieva,
Vladimir Gorbach,
Lubov Slepchenko,
Natalya Kirichuk,
Anna Podvolotskaya,
Oksana Son,
Liudmila Tekutyeva,
Larissa Balabanova

Affiliations

Iuliia Pentekhina: Institute of Biotechnology, Bioengineering and Food Systems, Advanced Engineering School, Far Eastern Federal University, 10 Ajax Bay, Russky Island, 690922 Vladivostok, Russia
Olga Nedashkovskaya: Laboratory of Marine Biochemistry, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Prospect 100-Letya Vladivostoka 152, 690022 Vladivostok, Russia
Aleksandra Seitkalieva: Institute of Biotechnology, Bioengineering and Food Systems, Advanced Engineering School, Far Eastern Federal University, 10 Ajax Bay, Russky Island, 690922 Vladivostok, Russia
Vladimir Gorbach: Laboratory of Marine Biochemistry, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Prospect 100-Letya Vladivostoka 152, 690022 Vladivostok, Russia
Lubov Slepchenko: Institute of Biotechnology, Bioengineering and Food Systems, Advanced Engineering School, Far Eastern Federal University, 10 Ajax Bay, Russky Island, 690922 Vladivostok, Russia
Natalya Kirichuk: Laboratory of Marine Biochemistry, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Prospect 100-Letya Vladivostoka 152, 690022 Vladivostok, Russia
Anna Podvolotskaya: Institute of Biotechnology, Bioengineering and Food Systems, Advanced Engineering School, Far Eastern Federal University, 10 Ajax Bay, Russky Island, 690922 Vladivostok, Russia
Oksana Son: Institute of Biotechnology, Bioengineering and Food Systems, Advanced Engineering School, Far Eastern Federal University, 10 Ajax Bay, Russky Island, 690922 Vladivostok, Russia
Liudmila Tekutyeva: Institute of Biotechnology, Bioengineering and Food Systems, Advanced Engineering School, Far Eastern Federal University, 10 Ajax Bay, Russky Island, 690922 Vladivostok, Russia
Larissa Balabanova: Institute of Biotechnology, Bioengineering and Food Systems, Advanced Engineering School, Far Eastern Federal University, 10 Ajax Bay, Russky Island, 690922 Vladivostok, Russia

DOI: https://doi.org/10.3390/microorganisms11092255
Journal volume & issue: Vol. 11, no. 9
p. 2255

Abstract

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Screening for chitinolytic activity in the bacterial strains from different Pacific Ocean regions revealed that the highly active representatives belong to the genera Microbulbifer, Vibrio, Aquimarina, and Pseudoalteromonas. The widely distributed chitinolytic species was Microbulbifer isolated from the sea urchin Strongylocentrotus intermedius. Among seventeen isolates with confirmed chitinolytic activity, only the type strain P. flavipulchra KMM 3630T and the strains of putatively new species Pseudoalteromonas sp. B530 and Vibrio sp. Sgm 5, isolated from sea water (Vietnam mollusc farm) and the sea urchin S. intermedius (Peter the Great Gulf, the Sea of Japan), significantly suppressed the hyphal growth of Aspergillus niger that is perspective for the biocontrol agents’ development. The results on chitinolytic activities and whole-genome sequencing of the strains under study, including agarolytic type strain Z. galactanivorans DjiT, found the new functionally active chitinase structures and biotechnological potential.

Published in Microorganisms

ISSN: 2076-2607 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General)
Website: https://www.mdpi.com/journal/microorganisms

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