Differentiation of beta-like cells from human induced pluripotent stem cell-derived pancreatic progenitor organoids

Abstract

Summary: Human induced pluripotent stem cells (hiPSCs) and organoids are important for modeling human development and disease in vitro. In this study, we describe a protocol to differentiate hiPSC toward pancreatic progenitor (PP) organoids and beta-like cells. We detail the expansion and seeding of hiPSC, PP differentiation, organoid expansion, and the differentiation of PP into beta cells. Upon differentiation, organoids contained beta, delta, and alpha cells.For complete details on the use and execution of this protocol, please refer to Cujba et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Developmental biology
• Metabolism
• Stem cells
• Cell differentiation
• Organoids