PURIFICATION AND BIOCHEMICAL CHARACTERIZATION OF IgG FROM SERUM OF YAK ( BOS GRUNNIENS )

Abstract

In the present study Immunoglobulin G was purified from serum of Yak by gel filtration chromatography on Sephacryl S-200. SDS- PAGE of purified yak IgG showed four major polypeptides of 62.94 kDa, 53.29 kDa 29.22 and 28.21 kDa. The purified Immunoglobulin has been found to be immuno-reactive by DID test and Western Blot analysis when treated against hyper-immune sera.

Keywords

• Immunoglobulin G
• Purification
• Gel filtration Chromatography
• Immunoreactive