Plant-Derived Exosomal Nanoparticles Inhibit Pathogenicity of Porphyromonas gingivalis

Kumaran Sundaram; Daniel P. Miller; Anil Kumar; Yun Teng; Mohammed Sayed; Jingyao Mu; Chao Lei; Mukesh K. Sriwastva; Lifeng Zhang; Yan Jun; Michael L. Merchant; Liqing He; Yuan Fang; Shuangqin Zhang; Xiang Zhang; Juw W. Park; Richard J. Lamont; Huang-Ge Zhang

iScience (Nov 2019)

Plant-Derived Exosomal Nanoparticles Inhibit Pathogenicity of Porphyromonas gingivalis

Kumaran Sundaram,
Daniel P. Miller,
Anil Kumar,
Yun Teng,
Mohammed Sayed,
Jingyao Mu,
Chao Lei,
Mukesh K. Sriwastva,
Lifeng Zhang,
Yan Jun,
Michael L. Merchant,
Liqing He,
Yuan Fang,
Shuangqin Zhang,
Xiang Zhang,
Juw W. Park,
Richard J. Lamont,
Huang-Ge Zhang

Affiliations

Kumaran Sundaram: James Graham Brown Cancer Center, Department of Microbiology & Immunology, University of Louisville, CTRB 309, 505 South Hancock Street, Louisville, KY 40202, USA
Daniel P. Miller: Department of Oral Immunology and Infectious Diseases, University of Louisville School of Dentistry, Louisville, KY 40202, USA
Anil Kumar: James Graham Brown Cancer Center, Department of Microbiology & Immunology, University of Louisville, CTRB 309, 505 South Hancock Street, Louisville, KY 40202, USA
Yun Teng: James Graham Brown Cancer Center, Department of Microbiology & Immunology, University of Louisville, CTRB 309, 505 South Hancock Street, Louisville, KY 40202, USA
Mohammed Sayed: Department of Computer Engineering and Computer Science, University of Louisville, Louisville, KY 40202, USA
Jingyao Mu: James Graham Brown Cancer Center, Department of Microbiology & Immunology, University of Louisville, CTRB 309, 505 South Hancock Street, Louisville, KY 40202, USA
Chao Lei: James Graham Brown Cancer Center, Department of Microbiology & Immunology, University of Louisville, CTRB 309, 505 South Hancock Street, Louisville, KY 40202, USA
Mukesh K. Sriwastva: James Graham Brown Cancer Center, Department of Microbiology & Immunology, University of Louisville, CTRB 309, 505 South Hancock Street, Louisville, KY 40202, USA
Lifeng Zhang: James Graham Brown Cancer Center, Department of Microbiology & Immunology, University of Louisville, CTRB 309, 505 South Hancock Street, Louisville, KY 40202, USA
Yan Jun: James Graham Brown Cancer Center, Department of Microbiology & Immunology, University of Louisville, CTRB 309, 505 South Hancock Street, Louisville, KY 40202, USA
Michael L. Merchant: Kidney Disease Program and Clinical Proteomics Center, University of Louisville, Louisville, KY, USA
Liqing He: Department of Pharmacology and Toxicology, University of Louisville, Louisville, KY 40202, USA
Yuan Fang: Department of Pharmacology and Toxicology, University of Louisville, Louisville, KY 40202, USA
Shuangqin Zhang: Department of Medicine, University of Chicago, Chicago, IL 60637, USA
Xiang Zhang: Department of Pharmacology and Toxicology, University of Louisville, Louisville, KY 40202, USA
Juw W. Park: Department of Computer Engineering and Computer Science, University of Louisville, Louisville, KY 40202, USA; KBRIN Bioinformatics Core, University of Louisville, Louisville, KY 40202, USA
Richard J. Lamont: Department of Oral Immunology and Infectious Diseases, University of Louisville School of Dentistry, Louisville, KY 40202, USA
Huang-Ge Zhang: Robley Rex Veterans Affairs Medical Center, Louisville, KY 40206, USA; James Graham Brown Cancer Center, Department of Microbiology & Immunology, University of Louisville, CTRB 309, 505 South Hancock Street, Louisville, KY 40202, USA; Corresponding author

Journal volume & issue: Vol. 21
pp. 308 – 327

Abstract

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Summary: Plant exosomes protect plants against infection; however, whether edible plant exosomes can protect mammalian hosts against infection is not known. In this study, we show that ginger exosome-like nanoparticles (GELNs) are selectively taken up by the periodontal pathogen Porphyromonas gingivalis in a GELN phosphatidic acid (PA) dependent manner via interactions with hemin-binding protein 35 (HBP35) on the surface of P. gingivalis. Compared with PA (34:2), PA (34:1) did not interact with HBP35, indicating that the degree of unsaturation of PA plays a critical role in GELN-mediated interaction with HBP35. On binding to HBP35, pathogenic mechanisms of P. gingivalis were significantly reduced following interaction with GELN cargo molecules, including PA and miRs. These cargo molecules interacted with multiple pathogenic factors in the recipient bacteria simultaneously. Using edible plant exosome-like nanoparticles as a potential therapeutic agent to prevent/treat chronic periodontitis was further demonstrated in a mouse model. : Bacteriology; Biochemistry; Biological Sciences; Immunology; Microbiology; Molecular Biology; Oral Microbiology Subject Areas: Bacteriology, Biochemistry, Biological Sciences, Immunology, Microbiology, Molecular Biology, Oral Microbiology

Published in iScience

ISSN: 2589-0042 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science
Website: http://www.cell.com/iscience/home

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