Biochemistry and Biophysics Reports (Dec 2020)

Ghrelin uses the GHS-R1a/Gi/cAMP pathway and induces differentiation only in mature osteoblasts. This ghrelin pathway is impaired in AIS patients

  • Ronan Barre,
  • Nicolas Beton,
  • Aurélie Batut,
  • Frank Accabled,
  • Jerome Sales de Gauzy,
  • Françoise Auriol,
  • Sanaa Eddiry,
  • Maithe Tauber,
  • Sara Laurencin,
  • Jean Pierre Salles,
  • Isabelle Gennero

Journal volume & issue
Vol. 24
p. 100782

Abstract

Read online

We have examined the Acylated Ghrelin (AG)/Gi pathway in different human osteoblastic cell lines. We have found that: 1) AG induces differentiation/mineralization only in mature osteoblasts; 2) the expression of GHS-R1a increases up to the mature cell stage, 3) the action is mediated via the GHS-R/Gi/cAMP pathway only in mature osteoblasts, and 4) osteoblastic cells from adolescent idiopathic scoliosis (AIS) are resistant to the AG/Gi/cAMP pathway. Altogether, these results suggested that AG uses the GHS-R1a/Gi/cAMP pathway to induce differentiation in mature osteoblasts only. This pathway is impaired in AIS osteoblasts. Understanding AG-specific pathways involved in normal and pathological osteoblasts may be useful for developing new treatments for pathologies such as AIS or osteoporosis.

Keywords