Generation of induced pluripotent stem cells from an HLA-B27 positive ankylosing spondylitis patient with syndesmophyte formation

Wan-Hao Tsai; Yen-Chun Lin; Shih-Hsun Lan; Yu-Heng Lin; Chun-Hsiung Chen; Song-Chou Hsieh; Chin-Hsiu Liu

Stem Cell Research (Oct 2024)

Generation of induced pluripotent stem cells from an HLA-B27 positive ankylosing spondylitis patient with syndesmophyte formation

Wan-Hao Tsai,
Yen-Chun Lin,
Shih-Hsun Lan,
Yu-Heng Lin,
Chun-Hsiung Chen,
Song-Chou Hsieh,
Chin-Hsiu Liu

Affiliations

Wan-Hao Tsai: Division of Allergy, Immunology & Rheumatology, Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan; Division of Immunology and Rheumatology, Fu Jen Catholic University Hospital, New Taipei City, Taiwan
Yen-Chun Lin: Division of Allergy, Immunology & Rheumatology, Department of Internal Medicine, National Taiwan University Hospital Yunlin Branch, Yunlin, Taiwan
Shih-Hsun Lan: Division of Allergy, Immunology & Rheumatology, Department of Internal Medicine, National Taiwan University Hospital Yunlin Branch, Yunlin, Taiwan
Yu-Heng Lin: Division of Allergy, Immunology & Rheumatology, Department of Internal Medicine, National Taiwan University Hospital Yunlin Branch, Yunlin, Taiwan
Chun-Hsiung Chen: Division of Allergy, Immunology and Rheumatology, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, New Taipei City, Taiwan; School of Medicine, Tzu Chi University, Hualien, Taiwan
Song-Chou Hsieh: Division of Allergy, Immunology & Rheumatology, Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan; College of Medicine, National Taiwan University, Taipei, Taiwan
Chin-Hsiu Liu: Division of Allergy, Immunology & Rheumatology, Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan; Division of Allergy, Immunology & Rheumatology, Department of Internal Medicine, National Taiwan University Hospital Yunlin Branch, Yunlin, Taiwan; College of Medicine, National Taiwan University, Taipei, Taiwan; Department of Medical Education and Research, National Taiwan University Hospital Yunlin Branch, Yunlin, Taiwan; Corresponding author at: No.7, Chung Shan S. Rd., Zhongzheng Dist., Taipei City 100225, Taiwan.

Journal volume & issue: Vol. 80
p. 103508

Abstract

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Human leukocyte antigen (HLA)-B27 is the genetic marker for ankylosing spondylitis (AS). Here, we generated induced pluripotent stem cells (iPSCs) from peripheral blood mononuclear cells of a male AS patient carrying HLA-B27 with syndesmophyte formation by using the Sendai-virus delivery system. The resulting iPSCs had a normal karyotype, expressed pluripotent markers, and could differentiate into three germ layers. This cellular model will provide a platform for studying pathological mechanisms of new bone formation in HLA-B27 positive AS patients.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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