Antibacterial Activity of Methanol Extract of Achillea Wilhe lmsii C. Koch Flower and Leaf

Abstract

Background and Objectives: Infection diseaseshave always been an important concern of human being and attracted theattention of a large number of professionals from the medical and laboratory community. On the other hand, antibiotic treatment can bring about microbial resistance and many side effects. Therefore, using medical plants with fewer side effects can be of great help in the treatment of these infections. The aim of this study was to examine antimicrobial effects of the flowers and leaves of methanol extract of Achilleawilhelmsii on the growth of some pathogenic bacteria.Methods: In this study, methanol extracts were obtained by Soxhlet apparatus and Rotary and were tested invitro against 4 bacteria species: Bacillus cereus, Staphylococcus aureus, Pseudomonas aeroginosa, Escherchia coli. Several concentrations of flowers and leaves of methanol extracts (20,30,50,400 mg/ml) were prepared by using DMSO solvent and then antimicrobial activity was tested using Agar well diffusion and dilution Test. Data were analyzed using Chi-square and AVONA statistical tests (p<0/001).Results: The results of this study showed that this plant had antimicrobial effects. Flower and leaves of methanol extracts had more inhibitory activity on Bacillus cereus and Staphylococcus aureus, On the other hand, weak inhibition was observed in the Escherchia coli and thus did not have any inhibitory effect on Pseudomonas aeroginosa. Conclusion: The results demonstrated that the flowers and leaves of methanol extracts of Achilleawilhelmsii have excellent antimicrobial effect on some of the bacteria. Therefore, its effect is better than that of selected antibiotics. However, we will be able to carry out researches for using this plant in the treatment of diseases.

Keywords

• Achilleawilhelmsii
• Anti-bacterial Agents
• Methanol