Nature Communications (Apr 2017)
A new inhibitor of the β-arrestin/AP2 endocytic complex reveals interplay between GPCR internalization and signalling
- Alexandre Beautrait,
- Justine S. Paradis,
- Brandon Zimmerman,
- Jenna Giubilaro,
- Ljiljana Nikolajev,
- Sylvain Armando,
- Hiroyuki Kobayashi,
- Lama Yamani,
- Yoon Namkung,
- Franziska M. Heydenreich,
- Etienne Khoury,
- Martin Audet,
- Philippe P. Roux,
- Dmitry B. Veprintsev,
- Stéphane A. Laporte,
- Michel Bouvier
Affiliations
- Alexandre Beautrait
- Department of Biochemistry, Institute for Research in Immunology and Cancer (IRIC), Université de Montréal
- Justine S. Paradis
- Department of Molecular Biology Program, Institute for Research in Immunology and Cancer (IRIC), Université de Montréal
- Brandon Zimmerman
- Department of Pharmacology and Therapeutics, Research Institute of McGill University Health Centre (RI-MUHC), McGill University
- Jenna Giubilaro
- Department of Pharmacology and Therapeutics, Research Institute of McGill University Health Centre (RI-MUHC), McGill University
- Ljiljana Nikolajev
- Department of Medicine, Research Institute of McGill University Health Centre (RI-MUHC), McGill University
- Sylvain Armando
- Department of Medicine, Research Institute of McGill University Health Centre (RI-MUHC), McGill University
- Hiroyuki Kobayashi
- Department of Biochemistry, Institute for Research in Immunology and Cancer (IRIC), Université de Montréal
- Lama Yamani
- Department of Medicine, Research Institute of McGill University Health Centre (RI-MUHC), McGill University
- Yoon Namkung
- Department of Medicine, Research Institute of McGill University Health Centre (RI-MUHC), McGill University
- Franziska M. Heydenreich
- Department of Biology and Chemistry, Laboratory of Biomolecular Research, Paul Scherrer Institut,5232 Villigen PSI, Switzerland, Switzerland
- Etienne Khoury
- Department of Medicine, Research Institute of McGill University Health Centre (RI-MUHC), McGill University
- Martin Audet
- Department of Biochemistry, Institute for Research in Immunology and Cancer (IRIC), Université de Montréal
- Philippe P. Roux
- Department of Pathology and Cellular Biology, Institute of Research in Immunology and Cancer (IRIC), Université de Montréal
- Dmitry B. Veprintsev
- Department of Biology and Chemistry, Laboratory of Biomolecular Research, Paul Scherrer Institut,5232 Villigen PSI, Switzerland, Switzerland
- Stéphane A. Laporte
- Department of Pharmacology and Therapeutics, Research Institute of McGill University Health Centre (RI-MUHC), McGill University
- Michel Bouvier
- Department of Biochemistry, Institute for Research in Immunology and Cancer (IRIC), Université de Montréal
- DOI
- https://doi.org/10.1038/ncomms15054
- Journal volume & issue
-
Vol. 8,
no. 1
pp. 1 – 16
Abstract
Beta-arrestins play central roles in the mechanisms regulating GPCR signalling and trafficking. Here the authors identify a selective inhibitor of the interaction between β-arrestin and the β2-adaptin subunit of the clathrin adaptor protein AP-2, which they use to dissect the role of the β-arrestin/β2-adaptin interaction in GPCR signalling.
