Draft genome of a biological control agent against Bipolaris sorokiniana, the causal phytopathogen of spot blotch in wheat (Triticum turgidum L. subsp. durum): Bacillus inaquosorum TSO22

Morales-Sandoval Pamela Helué; Valenzuela-Ruíz Valeria; Santoyo Gustavo; Hyder Sajjad; Mitra Debasis; Zelaya-Molina Lily X.; Ávila-Alistac Norma; Parra-Cota Fannie Isela; Santos-Villalobos Sergio de los

doi:10.1515/opag-2022-0309

Open Agriculture (Jun 2024)

Draft genome of a biological control agent against Bipolaris sorokiniana, the causal phytopathogen of spot blotch in wheat (Triticum turgidum L. subsp. durum): Bacillus inaquosorum TSO22

Morales-Sandoval Pamela Helué,
Valenzuela-Ruíz Valeria,
Santoyo Gustavo,
Hyder Sajjad,
Mitra Debasis,
Zelaya-Molina Lily X.,
Ávila-Alistac Norma,
Parra-Cota Fannie Isela,
Santos-Villalobos Sergio de los

Affiliations

Morales-Sandoval Pamela Helué: Facultad de Biología, Universidad Veracruzana, González Aguirre-Beltrán, 91090, Xalapa, Veracruz, Mexico
Valenzuela-Ruíz Valeria: Departamento de Ciencias Agronómicas y Veterinarias, Instituto Tecnológico de Sonora, 5 de febrero 818 sur, 85000, Ciudad Obregón, Sonora, Mexico
Santoyo Gustavo: Instituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, 58030, Morelia, Mexico
Hyder Sajjad: Department of Botany, Government College Women University, Sialkot, 51040, Pakistan
Mitra Debasis: Department of Microbiology, Graphic Era (Deemed to be University), 566/6, Bell Road, Clement Town Dehradun, 248002, Uttarakhand, India
Zelaya-Molina Lily X.: Centro Nacional de Recursos Genéticos, Insituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Blvd. de la Biodiversidad No. 400, C.P. 47600, Tepatitlán de Morelos, Jalisco, Mexico
Ávila-Alistac Norma: Departamento de Fitotecnia, Universidad Autónoma Chapingo, Km 38.5, Carretera México-Texcoco, Chapingo, Estado de México. C.P. 56230, Mexico
Parra-Cota Fannie Isela: Campo Experimental Norman E. Borlaug, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Norman E. Borlaug Km. 12, C. P. 85000, Cd. Obregón, Sonora, Mexico
Santos-Villalobos Sergio de los: Departamento de Ciencias Agronómicas y Veterinarias, Instituto Tecnológico de Sonora, 5 de febrero 818 sur, 85000, Ciudad Obregón, Sonora, Mexico

DOI: https://doi.org/10.1515/opag-2022-0309
Journal volume & issue: Vol. 9, no. 1
pp. 1000571 – 42

Abstract

Read online

Strain TSO22 was isolated from bulk soil associated with wheat crops in the Yaqui Valley, Mexico. The draft genome contained 4,294,671 bp, 43.7% G + C content, 731,759 bp N50, 3 L50, and 29 contigs. Based on 16S rRNA gene sequencing, the overall genome relatedness index, and phylogenomic relationship, strain TSO22 is highly affiliated with Bacillus inaquosorum. Genome annotation revealed 94 RNAs and 4,465 predicted coding DNA sequences distributed in 336 subsystems, in which genes involved in plant growth promotion and biocontrol were detected, such as colonizing plant system, competitive exclusionist, control of biotic and abiotic stresses, and plant immune response stimulation. Genomic predictions were confirmed through in vitro analysis, which revealed that B. inaquosorum TSO22 has great biological control against Bipolaris sorokiniana, as well as excellent metabolic capabilities to improve plant growth and development.

Published in Open Agriculture

ISSN: 2391-9531 (Online)
Publisher: De Gruyter
Country of publisher: Poland
LCC subjects: Agriculture: Agriculture (General)
Website: https://www.degruyter.com/view/j/opag

About the journal

Abstract

Keywords