CYTOTOXICITY STUDY OF FICUS RELIGIOSA LEAF EXTRACT  AGAINST JURKAT CELL LINE

Apratim Maity; P.C. Bisoi; A.B. Das; P. Maiti; M.R. Senapati

Exploratory Animal and Medical Research (Jun 2017)

CYTOTOXICITY STUDY OF FICUS RELIGIOSA LEAF EXTRACT AGAINST JURKAT CELL LINE

Apratim Maity,
P.C. Bisoi,
A.B. Das,
P. Maiti,
M.R. Senapati

Affiliations

Apratim Maity: Associate Professor, Department of Veterinary Biochemistry, West Bengal University of Animal and Fishery Sciences, West Bengal, India.
P.C. Bisoi: Department of Veterinary Biochemistry, College of Veterinary Science and Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar, Odisha., India.
A.B. Das: Associate Professor, Department of Agricultural Biotechnology, College of Agriculture, Orissa University of Agriculture and Technology, Bhubaneswar, Odisha., India.
P. Maiti: IMGENEX India Pvt. Ltd., Bhubaneswar, India.
M.R. Senapati: Department of Veterinary Biochemistry, College of Veterinary Science and Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar, Odisha., India.

Journal volume & issue: Vol. 7, no. 1
pp. 18 – 21

Abstract

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Different parts of Ficus religiosa tree have traditional usage against many ailments and diseases. In the present study, 85% ethanol extract of F. religiosa leaves were partially purified by PLC Silica gel 60 F254, 2 mm, 20X20 cm preparative thin layer chromatography using toluene and ethyl acetate (8:2, v/v) as solvent. Fraction 7 (FR7) showed highest brine shrimp larvicidal activity as regard to the leaf extract of F. religiosa. In vitro anticancer potential of same fraction against Jurkat cell line was investigated by means of flow cytometry using annexin V/7AAD apoptosis kit. F. religiosa leaf extract was found to markedly inhibit the proliferation of Jurkat cells in a dose-dependent manner.

Published in Exploratory Animal and Medical Research

ISSN: 2277-470X (Print); 2319-247X (Online)
Publisher: West Bengal Veterinary Alumni Association
Country of publisher: India
LCC subjects: Agriculture: Animal culture: Veterinary medicine
Website: https://animalmedicalresearch.org/

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