CRISPR-clear imaging of melanin-rich B16-derived solid tumors

Rajib Schubert; Taegeun Bae; Branko Simic; Sheena N. Smith; Seong-Ho Park; Gabriela Nagy-Davidescu; Viviana Gradinaru; Andreas Plückthun; Junho K. Hur

doi:10.1038/s42003-023-04614-7

Communications Biology (Apr 2023)

CRISPR-clear imaging of melanin-rich B16-derived solid tumors

Rajib Schubert,
Taegeun Bae,
Branko Simic,
Sheena N. Smith,
Seong-Ho Park,
Gabriela Nagy-Davidescu,
Viviana Gradinaru,
Andreas Plückthun,
Junho K. Hur

Affiliations

Rajib Schubert: Department of Biochemistry, University of Zürich
Taegeun Bae: Department of Medicine, Graduate School, Kyung Hee University
Branko Simic: Department of Biochemistry, University of Zürich
Sheena N. Smith: Department of Biochemistry, University of Zürich
Seong-Ho Park: Department of Medicine, Major in Medical Genetics, Graduate School, Hanyang University
Gabriela Nagy-Davidescu: Department of Biochemistry, University of Zürich
Viviana Gradinaru: Division of Biology and Biological Engineering, California Institute of Technology
Andreas Plückthun: Department of Biochemistry, University of Zürich
Junho K. Hur: Department of Genetics, College of Medicine, Hanyang University

DOI: https://doi.org/10.1038/s42003-023-04614-7
Journal volume & issue: Vol. 6, no. 1
pp. 1 – 8

Abstract

Read online

Tyrosinase CRISPR knock out in the B16 melanoma cell line eliminates melanin production, with the lack of pigment not affecting tumour engraftment or growth and allowing for 3D imaging after clearing by PACT.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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