Microbiome characterization by high-throughput transfer RNA sequencing and modification analysis

Michael H. Schwartz; Haipeng Wang; Jessica N. Pan; Wesley C. Clark; Steven Cui; Matthew J. Eckwahl; David W. Pan; Marc Parisien; Sarah M. Owens; Brian L. Cheng; Kristina Martinez; Jinbo Xu; Eugene B. Chang; Tao Pan; A. Murat Eren

doi:10.1038/s41467-018-07675-z

Nature Communications (Dec 2018)

Microbiome characterization by high-throughput transfer RNA sequencing and modification analysis

Michael H. Schwartz,
Haipeng Wang,
Jessica N. Pan,
Wesley C. Clark,
Steven Cui,
Matthew J. Eckwahl,
David W. Pan,
Marc Parisien,
Sarah M. Owens,
Brian L. Cheng,
Kristina Martinez,
Jinbo Xu,
Eugene B. Chang,
Tao Pan,
A. Murat Eren

Affiliations

Michael H. Schwartz: Department of Biochemistry and Molecular Biology, University of Chicago
Haipeng Wang: Department of Biochemistry and Molecular Biology, University of Chicago
Jessica N. Pan: Department of Biochemistry and Molecular Biology, University of Chicago
Wesley C. Clark: Department of Biochemistry and Molecular Biology, University of Chicago
Steven Cui: Department of Medicine, University of Chicago
Matthew J. Eckwahl: Department of Biochemistry and Molecular Biology, University of Chicago
David W. Pan: Department of Biochemistry and Molecular Biology, University of Chicago
Marc Parisien: Department of Biochemistry and Molecular Biology, University of Chicago
Sarah M. Owens: Department of Medicine, University of Chicago
Brian L. Cheng: Committee on Microbiology, University of Chicago
Kristina Martinez: Department of Medicine, University of Chicago
Jinbo Xu: Toyota Technological Institute at Chicago
Eugene B. Chang: Department of Medicine, University of Chicago
Tao Pan: Department of Biochemistry and Molecular Biology, University of Chicago
A. Murat Eren: Committee on Microbiology, University of Chicago

DOI: https://doi.org/10.1038/s41467-018-07675-z
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 13

Abstract

Read online

The authors present a molecular approach and a software suite for high-throughput sequencing and characterization of microbial tRNA transcripts, which provide unique physiological insights into complex environmental microbes.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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