Association of EV71 3C polymorphisms with clinical severity

Hsuan-Yin Ma; Chun-Yi Lu; Kuo-Chien Tsao; Hsiu-Ming Shih; Ai-Ling Cheng; Li-Min Huang; Luan-Yin Chang

Journal of Microbiology, Immunology and Infection (Oct 2018)

Association of EV71 3C polymorphisms with clinical severity

Hsuan-Yin Ma,
Chun-Yi Lu,
Kuo-Chien Tsao,
Hsiu-Ming Shih,
Ai-Ling Cheng,
Li-Min Huang,
Luan-Yin Chang

Affiliations

Hsuan-Yin Ma: Division of Pediatric Infectious Diseases, Department of Pediatrics, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan
Chun-Yi Lu: Division of Pediatric Infectious Diseases, Department of Pediatrics, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan
Kuo-Chien Tsao: Department of Medical Biotechnology, Chang Gung University, Taoyuan, Taiwan
Hsiu-Ming Shih: Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan
Ai-Ling Cheng: Division of Pediatric Infectious Diseases, Department of Pediatrics, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan
Li-Min Huang: Division of Pediatric Infectious Diseases, Department of Pediatrics, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan; Corresponding author. Division of Pediatric Infectious Diseases, Department of Pediatrics, National Taiwan University Hospital, College of Medicine, National Taiwan University, No. 8, Chung-Shan South Road, Taipei 100, Taiwan.
Luan-Yin Chang: Division of Pediatric Infectious Diseases, Department of Pediatrics, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan; Corresponding author. Division of Pediatric Infectious Diseases, Department of Pediatrics, National Taiwan University Hospital, College of Medicine, National Taiwan University, No. 8, Chung-Shan South Road, Taipei 100, Taiwan. Fax: +886 2 23147450.

Journal volume & issue: Vol. 51, no. 5
pp. 608 – 613

Abstract

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Objectives: Enterovirus 71 (EV71) may cause neurological and fatal cases. EV71 3C plays an important role on viral replication and possess proteolysis activity. To delineate pathogenesis of EV71 virulence, we studied EV71 3C genetics, protease activity and correlated the results with clinical severity. Methods: EV71 cases were collected; 3C of EV71 was sequenced and linked with clinical severity. 3C protease activity, viral replication rates of EV71 infectious clones with different 3C and 3C interaction with host proteins were analyzed. Results: The polymorphisms of EV71 3C at the 79th amino acid were associated with clinical severity. About 26% (62/234) patients infected by EV71 with wild-type 3C (T79) had neurological involvement but 78% (25/32) patients infected by EV71 with mutant 3C (T79V) did (p < 0.001). There was no significant difference of protease activity among the different 3C variants. EV71 with mutant 3C (T79V) had the highest viral replication rate and the mutant 3C (T79V) had weaker interaction with TRIM21, a component of antibody-dependent intracellular neutralization, than the other mutants (T79I and T79A). Conclusion: We found that 3C polymorphisms were associated with clinical severity and viral replication, which might be related to 3C interaction with important host proteins such as TRIM21. Keywords: Enterovirus 71, 3C protease, Pathogenesis, Virulence, TRIM21, Replication

Published in Journal of Microbiology, Immunology and Infection

ISSN: 1684-1182 (Print)
Publisher: Elsevier
Country of publisher: Hong Kong
LCC subjects: Science: Microbiology
Website: https://www.sciencedirect.com/journal/journal-of-microbiology-immunology-and-infection

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